Division of Fruit Crops, ICAR-Indian Institute of Horticultural Research, Bangalore, 560089, India.
Division of Fruit Crops, ICAR-Indian Institute of Horticultural Research, Bangalore, 560089, India.
Cryobiology. 2021 Feb;98:112-118. doi: 10.1016/j.cryobiol.2020.11.017. Epub 2020 Dec 4.
Cryopreservation of pollen is a complementary conservation strategy and can be used for conserve the diversity in the genus Psidium. The present study aims to cryopreserve the pollen of Psidium species to overcome asynchronous flowering. The pollen of different Psidium species were germinated in vitro in an optimized medium of germination. In vitro/in vivo pollen viability assessment and SEM analysis were carried out to determine the changes after cryopreservation. The in vitro pollen viability was determined at monthly intervals starting from fresh pollen until six months of cryopreservation. The in vivo fertility tests were carried out by pollination using both fresh and cryopreserved pollen. The cryopreserved pollen showed in vitro germination ranging from 1.78% (in P. molle) to 81.67% (in "H 12-5") compared to fresh pollen (2.16% in P. molle to 86.08% in P. guineense). In vivo fertility was tested by controlled pollination using six-month-old cryopreserved pollen and it resulted in fruit setting ranged between 3.33% (P. cattleianum var. cattleianum) and 27.66% (P. chinensis) as compared to fresh pollen between 4.0% (P. cattleianum var. cattleianum) and 30.66% (P. chinensis). Seed set and germination was also recorded in all the crosses attempted using cryopreserved pollen. These in vitro and in vivo results indicated that cryopreservation is an effective technique for breeding and conserving the haploid gene pool in cryo genebank. Scanning Electron Microscopic studies of pollen revealed no significant variation in shape and size after cryopreservation when compared to fresh pollen.
花粉的低温保存是一种补充性的保护策略，可用于保护Psidium 属的多样性。本研究旨在通过低温保存 Psidium 物种的花粉来克服开花不同步的问题。在优化的萌发培养基中对不同 Psidium 物种的花粉进行了离体萌发，进行了离体/体内花粉活力评估和 SEM 分析，以确定低温保存后的变化。从新鲜花粉开始，每月间隔一次测定体外花粉活力，直到低温保存六个月。通过使用新鲜花粉和低温保存花粉进行授粉进行了体内生育力测试。与新鲜花粉相比（P. molle 中的 2.16%，P. guineense 中的 86.08%），低温保存花粉的离体萌发率在 1.78%（P. molle 中）到 81.67%（"H 12-5"中）之间。通过使用六个月龄的低温保存花粉进行控制性授粉测试了体内生育力，结果表明结实率在 3.33%（P. cattleianum var. cattleianum）到 27.66%（P. chinensis）之间，而新鲜花粉的结实率在 4.0%（P. cattleianum var. cattleianum）到 30.66%（P. chinensis）之间。在所有使用低温保存花粉进行的杂交中也记录了种子的形成和萌发。这些离体和体内结果表明，低温保存是一种有效的技术，可以用于培育和保护冷冻基因库中的单倍体基因库。与新鲜花粉相比，低温保存后花粉的形状和大小的扫描电子显微镜研究没有发现明显的变化。
