Nagar Arvind, Gowthami Ravi, Sureja Amish Kumar, Munshi Anilabha Das, Verma Manjusha, Singh Awani Kumar, Mallick Niharika, Singh Jogendra, Chander Subhash, Shankar Muthusamy, Pathania Pooja, Rajkumar Subramani
Krishi Vigyan Kendra (KVK), Jhalawar, Agricultural University, Kota, India.
Division of Germplasm Conservation, ICAR-National Bureau of Plant Genetic Resources, New Delhi, India.
Front Plant Sci. 2023 Oct 27;14:1268726. doi: 10.3389/fpls.2023.1268726. eCollection 2023.
This study aimed to develop a long-term pollen storage protocol for species (, , , and ) and assess its potential for crop improvement. The optimal medium for pollen germination varied by species, with Brewbaker and Kwack (BK) medium with 10% sucrose suitable for , and , and BK medium with 3% sucrose ideal for . Overestimation in staining tests compared to pollen germination was observed. The best results for cryopreservation were achieved with desiccation periods of 20, 30, and 40 min, maintaining moisture content between 14.04% and 18.55%. Pollen viability was negatively correlated with storage temperature (25, 4, and -20°C) and duration. Cryopreserved pollen at -196°C exhibited the highest viability over a prolonged period (2 months) and was comparable to fresh pollen in terms of germination, ovule fertilization, and fruit and seed set. This study presents a simple and reproducible pollen cryopreservation protocol applicable across species, facilitating long-term storage and its use in crop improvement efforts.
本研究旨在为多种物种（、、和）制定长期花粉保存方案，并评估其在作物改良方面的潜力。适合花粉萌发的最佳培养基因物种而异，含10%蔗糖的Brewbaker和Kwack（BK）培养基适用于和，含3%蔗糖的BK培养基对最为理想。与花粉萌发相比，染色试验中存在高估现象。干燥20、30和40分钟，使水分含量保持在14.04%至18.55%之间时，冷冻保存取得了最佳效果。花粉活力与储存温度（25、4和-20°C）及储存时间呈负相关。在-196°C下冷冻保存的花粉在较长时期（2个月）内表现出最高活力，在萌发、胚珠受精以及果实和种子结实方面与新鲜花粉相当。本研究提出了一种适用于多种物种的简单且可重复的花粉冷冻保存方案，便于长期保存及其在作物改良工作中的应用。
