National Engineering Laboratory of Tree Breeding, Beijing Forestry University, 100083, China.
National Engineering Laboratory of Tree Breeding, Beijing Forestry University, 100083, China.
Int J Biol Macromol. 2018 Jul 1;113:655-661. doi: 10.1016/j.ijbiomac.2018.02.081. Epub 2018 Mar 6.
Heavy metal ATPase (HMA) plays an important role in phytoremediation via long-distance transportation from root to shoot. In this report, we identified a heavy metal ATPase gene, PtoHMA5, from Populus tomentosa Carr. Its encoded peptide consists of 967 amino acids and has eight trans-membrane motifs inside. Tobacco plants were transformed with this gene via Agrobacterium tumefaciens-mediated method. After exposure to 50mg/LCdCl for 10d, the transgenic lines displayed higher cadmium accumulation in leaves than did the wild-type plants with an absolute increase of 25.04%, while the transfer coefficient increased by 16.01%-43.25%. Physiological testing including assessment of relative electrolytic leakage (REL), malondialdehyde (MDA) content, and chlorophyll content revealed that the transgenic lines were seriously affected when compared with the wild-type plants. In summary, PtoHMA5 is really involved in cadmium transport from root to shoot but is not associated with the removal of cadmium toxicity.
重金属 ATP 酶（HMA）在植物修复中通过从根部到地上部的远距离运输发挥着重要作用。在本报告中，我们从毛白杨中鉴定出一个重金属 ATP 酶基因 PtoHMA5，其编码的肽由 967 个氨基酸组成，内部有八个跨膜基序。通过根癌农杆菌介导的方法将该基因转化到烟草植物中。在暴露于 50mg/L CdCl 10d 后，与野生型植物相比，转基因系在叶片中积累了更高的镉，绝对增加了 25.04%，而转移系数增加了 16.01%-43.25%。包括相对电导率（REL）、丙二醛（MDA）含量和叶绿素含量在内的生理测试表明，与野生型植物相比，转基因系受到了严重影响。总之，PtoHMA5 确实参与了镉从根部到地上部的运输，但与镉毒性的去除无关。
