Liang Yan, Gao Qisong, Zhang Qiang, Arbab Abdelaziz Adam Idriss, Li Mingxun, Yang Zhangping, Karrow Niel A, Mao Yongjiang
Key Laboratory for Animal Genetics, Breeding, Reproduction and Molecular Design of Jiangsu Province, College of Animal Science and Technology, Yangzhou University, Yangzhou 225009, China.
Joint International Research Laboratory of Agriculture and Agri-Product Safety of Ministry of Education of China, Yangzhou University, Yangzhou 225009, China.
Animals (Basel). 2020 Dec 3;10(12):2282. doi: 10.3390/ani10122282.
Improving the quality of milk is a challenge for zootechnicians and dairy farms across the globe. Long-chain acyl-CoA synthetase 1 () is a significant member of the long-chain acyl-CoA synthetase gene family. It is widely found in various organisms and influences the lactation performance of cows, including fat percentage, milk protein percentage etc. Our study was aimed to investigate the genetic effects of single nucleotide polymorphisms (SNPs) in on milk production traits. Twenty Chinese Holstein cows were randomly selected to extract DNA from their blood samples for PCR amplification and sequencing to identify SNPs of the bovine gene, and six SNPs (5'UTR-g.20523C>G, g.35446C>T, g.35651G>A, g.35827C>T, g.35941G>A and g.51472C>T) were discovered. Then, Holstein cow genotyping (n = 992) was performed by Sequenom MassARRAY based on former SNP information. Associations between SNPs and milk production traits and somatic cell score (SCS) were analyzed by the least-squares method. The results showed that SNP g.35827C>T was in high linkage disequilibrium with g.35941G>A. Significant associations were found between SNPs and test-day milk yield (TDMY), fat content (FC), protein content (PC) and SCS ( < 0.05). Among these SNPs, SNP 5'UTR-g.20523C>G showed an extremely significant effect on PC and SCS ( < 0.01). The SNP g.35446C>T showed a statistically significant effect on FC, PC, and SCS ( < 0.01), and also TDMY ( < 0.05). The SNP g.35651G>A had a statistically significant effect on PC ( < 0.01). The SNP g.35827C>T showed a highly significant effect on TDMY, FC, and SCS ( < 0.01) and significantly influenced PC ( < 0.05). Lastly, SNP g.51472C>T was significantly associated with TDMY, FC, and SCS ( < 0.05). In summary, the pleiotropic effects of bovine for milk production traits were found in this paper, but further investigation will be required on the intrinsic correlation to provide a theoretical basis for the research on molecular genetics of milk quality traits of Holstein cows.
提高牛奶质量是全球畜牧技术人员和奶牛场面临的一项挑战。长链脂酰辅酶A合成酶1（）是长链脂酰辅酶A合成酶基因家族的重要成员。它广泛存在于各种生物体中，并影响奶牛的泌乳性能，包括乳脂率、乳蛋白率等。我们的研究旨在调查基因单核苷酸多态性（SNPs）对产奶性状的遗传效应。随机选取20头中国荷斯坦奶牛，从其血液样本中提取DNA进行PCR扩增和测序，以鉴定牛基因的SNPs，共发现6个SNPs（5'UTR - g.20523C>G、g.35446C>T、g.35651G>A、g.35827C>T、g.35941G>A和g.51472C>T）。然后，根据先前的SNP信息，采用Sequenom MassARRAY对荷斯坦奶牛进行基因分型（n = 992）。采用最小二乘法分析SNPs与产奶性状和体细胞评分（SCS）之间的关联。结果表明，SNP g.35827C>T与g.35941G>A处于高度连锁不平衡状态。发现SNPs与测定日产奶量（TDMY）、脂肪含量（FC）、蛋白质含量（PC）和SCS之间存在显著关联（<0.05）。在这些SNPs中，SNP 5'UTR - g.20523C>G对PC和SCS有极显著影响（<0.01）。SNP g.35446C>T对FC、PC和SCS有统计学显著影响（<0.01），对TDMY也有显著影响（<0.05）。SNP g.35651G>A对PC有统计学显著影响（<0.01）。SNP g.35827C>T对TDMY、FC和SCS有高度显著影响（<0.01），对PC有显著影响（<0.05）。最后，SNP g.51472C>T与TDMY、FC和SCS显著相关（<0.05）。综上所述,本文发现了牛基因对产奶性状的多效性，但内在相关性还需要进一步研究，为荷斯坦奶牛乳品质性状分子遗传学研究提供理论依据。
