Shandong Provincial Key Laboratory of Applied Microbiology, Ecology Institute, Qilu University of Technology (Shandong Academy of Sciences), Jinan, Shandong 250013, People's Republic of China.
Shandong Provincial Key Laboratory of Biophysics, Institute of Biophysics, Dezhou University, Dezhou 253023, People's Republic of China.
J Agric Food Chem. 2020 Dec 16;68(50):14832-14840. doi: 10.1021/acs.jafc.0c05746. Epub 2020 Dec 7.
Phenazine-1-carboxylic acid (PCA), the primary active ingredient of Shenqinmycin, was awarded the China Pesticide Certificate in 2011 due to its excellent antibacterial action. Phenazine-1-carboxamide (PCN) is a derivative of PCA, which is modified by the gene, and its anti-bacterial effect is better than that of PCA. At present, PCN can be produced via fermentation using an opportunistic pathogen, . Qlu-1 is an environmentally friendly strain of that can produce phenazine derivatives. We replaced the gene with the gene from to achieve PCN accumulation. Different strategies were used to enhance PCN production: knocking out of negative regulatory factors, enhancing the shikimate pathway by gene overexpression and gene knocking, and using fed-batch fermentation. Finally, an engineered strain of was produced, which produced 11.45 g/L PCN. This achievement indicates that Qlu-1 could be modified as a potential microbial cell factory for PCN production by metabolic engineering.
吩嗪-1-羧酸（PCA）是申嗪霉素的主要活性成分，由于其具有优异的抗菌作用，于 2011 年获得中国农药证书。吩嗪-1-甲酰胺（PCN）是 PCA 的衍生物，通过基因修饰，其抗菌效果优于 PCA。目前，PCN 可以通过一种机会性病原体发酵生产，该病原体为。Qlu-1 是一种环保型，能够产生吩嗪衍生物。我们用来自的基因替换了基因，实现了 PCN 的积累。我们采用了不同的策略来提高 PCN 的产量：敲除负调控因子，通过基因过表达和基因敲除增强莽草酸途径，以及使用分批补料发酵。最终，我们生产出了一株工程菌，其 PCN 产量达到了 11.45 g/L。这一成果表明，通过代谢工程，Qlu-1 可以被修饰为生产 PCN 的潜在微生物细胞工厂。
