School of Rehabilitation Medicine, Capital Medical University, Beijing, PR China.
China Rehabilitation Science Institute, Beijing, PR China.
Acta Radiol. 2021 Dec;62(12):1666-1673. doi: 10.1177/0284185120975182. Epub 2020 Dec 7.
Spinal cord injury (SCI) is a severe central nervous system injury that can generally induce different degrees of sensory and motor dysfunction.
To clarify the changes of diffusion tensor imaging (DTI) parameters after spinal cord myelotomy in rats with SCI.
Eighteen Sprague Dawley (SD) rats were randomly divided into the Sham group (n=6), SCI group (n=6), and Mye group (n=6), respectively. The DTI values at 1, 3, 7, and 21 days after modeling were collected by magnetic resonance imaging (MRI). The spinal specimen at the injury site was collected on the 21st day for Nissl's staining to assess the changes in neurons.
The fractional anisotropy (FA) values in both the SCI group and Mye group significantly decreased. In addition, the FA values between the two groups were statistically significant ( < 0.001). The apparent diffusion coefficient (ADC), mean diffusivity (MD), axial diffusivity (AD), and radial diffusivity (RD) values all decreased and then increased ( < 0.001). Pearson correlation test showed that the ADC, MD, and AD values were positively correlated with the Basso Beattie Bresnahan (BBB) score. Nissl's staining showed a higher number of Nissl's bodies, and deep staining of Nissl's bodies in the Mye group, while the morphology of neurons was relatively good. The number of neurons in the Mye group was significantly higher after myelotomy compared to the SCI group ( < 0.001).
The DTI parameters, especially ADC values, could non-invasively and quantifiably evaluate the efficacy of myelotomy for rats with SCI.
脊髓损伤（SCI）是一种严重的中枢神经系统损伤，通常会导致不同程度的感觉和运动功能障碍。
明确 SCI 大鼠脊髓切开术后弥散张量成像（DTI）参数的变化。
18 只 Sprague Dawley（SD）大鼠随机分为假手术组（n=6）、SCI 组（n=6）和切开组（n=6）。通过磁共振成像（MRI）分别在建模后 1、3、7 和 21 天采集 DTI 值。在第 21 天收集损伤部位的脊髓标本进行尼氏染色，以评估神经元的变化。
SCI 组和切开组的各向异性分数（FA）值均显著降低。此外，两组间 FA 值存在统计学差异（ < 0.001）。表观扩散系数（ADC）、平均扩散系数（MD）、轴突扩散系数（AD）和径向扩散系数（RD）均降低后升高（ < 0.001）。Pearson 相关检验显示，ADC、MD 和 AD 值与 Basso Beattie Bresnahan（BBB）评分呈正相关。尼氏染色显示切开组尼氏体数量较多，尼氏体深染，神经元形态相对较好。切开组神经元数量明显高于 SCI 组（ < 0.001）。
DTI 参数，特别是 ADC 值，可无创、定量地评估脊髓切开术对 SCI 大鼠的疗效。
