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扩散张量成像评估挫伤性脊髓损伤（SCI）后嗅鞘细胞移植大鼠功能恢复的可行性

Feasibility of Diffusion Tensor Imaging for Assessing Functional Recovery in Rats with Olfactory Ensheathing Cell Transplantation After Contusive Spinal Cord Injury (SCI).

作者信息

Gu Mengchao, Gao Zhengchao, Li Xiaohui, Zhao Feng, Guo Lei, Liu Jiantao, He Xijing

机构信息

Department of Othopaedics, Second Affiliated Hospital of Xi'an Jiaotong University, Xi'an, Shaanxi, China (mainland).

Department of Radiology, Second Affiliated Hospital of Xi'an Jiaotong University, Xi'an, Shaanxi, China (mainland).

出版信息

Med Sci Monit. 2017 Jun 17;23:2961-2971. doi: 10.12659/msm.902126.

DOI:10.12659/msm.902126
PMID:28623671
原文链接:https://pmc.ncbi.nlm.nih.gov/articles/PMC5484594/
Abstract

BACKGROUND Olfactory ensheathing cell transplantation is a promising treatment for spinal cord injury. Diffusion tensor imaging has been applied to assess various kinds of spinal cord injury. However, it has rarely been used to evaluate the beneficial effects of olfactory ensheathing cell transplantation. This study aimed to explore the feasibility of diffusion tensor imaging in the evaluation of functional recovery in rats with olfactory ensheathing cell transplantation after contusive spinal cord injury. MATERIAL AND METHODS Immunofluorescence staining was performed to determine the purity of olfactory ensheathing cells. Rats received cell transplantation at week 1 after injury. Basso, Beattie, and Bresnahan score was used to assess the functional recovery. Magnetic resonance imaging was applied weekly, including diffusion tensor imaging. Diffusion tensor tractography was reconstructed to visualize the repair process. RESULTS The results showed that olfactory ensheathing cell transplantation increased the functional and histological recovery and restrained the secondary injury process after the initial spinal cord injury. The fractional anisotropy values in rats with cell transplantation were significantly higher than those in the control group, while the apparent diffusion coefficient values were significantly lower. Basso, Beattie, and Bresnahan score was positively and linearly correlated with fractional anisotropy value, and it was negatively and linearly correlated with apparent diffusion coefficient value. CONCLUSIONS These findings suggest that diffusion tensor imaging parameters are sensitive biomarker indices for olfactory ensheathing cell transplantation interventions, and diffusion tensor imaging scan can reflect the functional recovery promoted by the olfactory ensheathing cell transplantation after contusive spinal cord injury.

摘要

背景

嗅鞘细胞移植是一种有前景的脊髓损伤治疗方法。扩散张量成像已被应用于评估各种脊髓损伤。然而，它很少被用于评估嗅鞘细胞移植的有益效果。本研究旨在探讨扩散张量成像在评估脊髓挫伤性损伤后接受嗅鞘细胞移植大鼠功能恢复方面的可行性。

材料与方法

进行免疫荧光染色以确定嗅鞘细胞的纯度。大鼠在损伤后第1周接受细胞移植。使用Basso、Beattie和Bresnahan评分来评估功能恢复情况。每周进行磁共振成像，包括扩散张量成像。重建扩散张量纤维束成像以可视化修复过程。

结果

结果表明，嗅鞘细胞移植增加了功能和组织学恢复，并抑制了初始脊髓损伤后的继发性损伤过程。细胞移植大鼠的分数各向异性值显著高于对照组，而表观扩散系数值显著更低。Basso、Beattie和Bresnahan评分与分数各向异性值呈正线性相关，与表观扩散系数值呈负线性相关。

结论

这些发现表明，扩散张量成像参数是嗅鞘细胞移植干预的敏感生物标志物指标，扩散张量成像扫描可以反映脊髓挫伤性损伤后嗅鞘细胞移植促进的功能恢复。

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