Division of Animal and Dairy Sciences, College of Agriculture and Life Sciences, Chungnam National University, Daejeon, Republic of Korea.
Livestock Experiment Institute, Government of Chungcheongnam-do, Chungcheongnam-do, Republic of Korea.
Anim Biotechnol. 2022 Oct;33(5):964-969. doi: 10.1080/10495398.2020.1856124. Epub 2020 Dec 7.
Genome-wide association studies (GWAS) have accelerated the identification of functional trait loci in cattle and identified single nucleotide polymorphisms (SNPs) in candidate genes associated with fertility and production traits in high milk yield dairy cattle. The fertility of Hanwoo (Korean native beef cattle) has declined after the adaptation of a selection program for high quantity and quality meat. However, there are few GWAS studies of fertility in beef cattle. We performed a genome-wide association study of 40 Korean native beef cattle heifers with imputed 770 K genotype and identified 12 significant SNPs within seven regions on three chromosomes (BTA 8, BTA 16 and BTA 24) associated with services per conception (SPC). Five SNPs were located in the , and genes, which are involved in early embryo development. In addition, 27 proximal genes were identified within 1 Mb of the candidate SNPs, which are involved in muscle cell differentiation and muscle structure development. However, we did not find any previously reported SNPs related to fertility in Holstein cows. Taken together, we identified SNPs associated with SPC and their proximal genes using gene-based analysis and the candidates were different from SNPs associated with subfertility of dairy cattle.
全基因组关联研究（GWAS）加速了牛的功能性状基因座的鉴定，并鉴定了与高产奶牛产奶和生产性状相关的候选基因中的单核苷酸多态性（SNP）。韩牛（韩国本土肉牛）在适应了高数量和高质量肉类的选择计划后，其繁殖力下降。然而，关于肉牛繁殖力的 GWAS 研究很少。我们对 40 头韩国本土肉牛小母牛进行了全基因组关联研究，这些小母牛的基因型被推断为 770 K，并在三个染色体（BTA 8、BTA 16 和 BTA 24）上的七个区域中鉴定出与受胎率（SPC）相关的 12 个显著 SNP。五个 SNP 位于 、 和 基因中，这些基因参与早期胚胎发育。此外，在候选 SNP 的 1 Mb 内还鉴定出 27 个近端基因，这些基因参与肌肉细胞分化和肌肉结构发育。然而，我们没有发现任何先前报道的与荷斯坦奶牛生育能力相关的 SNP。综上所述，我们使用基于基因的分析鉴定了与 SPC 相关的 SNP 及其近端基因，候选 SNP 与奶牛不孕不育相关的 SNP 不同。
