Schnier J
Max-Planck-Institut für Molekulare Genetik, Abt. Wittmann, Berlin, FRG.
J Gen Microbiol. 1987 Nov;133(11):3151-8. doi: 10.1099/00221287-133-11-3151.
Various conditions which decrease translational capacity and enhance the synthesis of ribosomal components were analysed with respect to the synthesis of heat-shock proteins in Escherichia coli: (a) deprivation of streptomycin from a streptomycin-dependent mutant, (b) addition of tetracycline to a partially tetracycline-resistant strain, and (c) nutritional shift-up conditions. In all cases, the rate of synthesis of the heat-shock proteins DnaK, GroEL and C62.5 decreased while the synthesis of ribosomal components increased. Thus inhibition of ribosome formation or a decrease in translational capacity do not induce the stress proteins, but have the opposite effect.
针对大肠杆菌中热休克蛋白的合成，分析了各种降低翻译能力并增强核糖体组分合成的条件：(a) 从链霉素依赖型突变体中去除链霉素；(b) 向部分耐四环素菌株中添加四环素；(c) 营养上调条件。在所有情况下，热休克蛋白DnaK、GroEL和C62.5的合成速率均下降，而核糖体组分的合成增加。因此，抑制核糖体形成或降低翻译能力并不会诱导应激蛋白的产生，反而会产生相反的效果。
