CAS Key Laboratory of Plant Germplasm Enhancement and Specialty Agriculture, Wuhan Botanical Garden, Chinese Academy of Sciences, Wuhan, 430074, China; University of Chinese Academy of Sciences, Beijing, 100049, China; Center of Economic Botany, Core Botanical Gardens, Chinese Academy of Sciences, Wuhan, 430074, China.
State Key Laboratory of Rice Biology, Zhejiang Provincial Key Laboratory of Crop Genetic Resources, Institute of Crop Science, Zhejiang University, Hangzhou, 310058, China.
Plant Sci. 2021 Jan;302:110677. doi: 10.1016/j.plantsci.2020.110677. Epub 2020 Oct 9.
In higher plants, floral signals are mainly collected and transduced to FLOWERING LOCUS T (FT) in Arabidopsis and its orthologues. The movement of FT from leaves to the shoot apical meristem (SAM) is partially mediated by FT-INTERACTING PROTEIN1 (FTIP1). Although the functions of OsFTIP1 in rice and DOFTIP1 in orchid in FT transport have also been investigated, the FTIP1 homologue in lotus (Nelumbo nucifera Gaertn.), a type of horticultural plant with high economic and cultural value, has not been isolated, and the mechanism of NnFT1 transport has not been explored. Here, we revealed that NnFTIP1 mediates the transport of NnFT1 in ectopic transgenic lines in Arabidopsis. Overexpression of NnFTIP1 in the ftip1-1 background rescued the late flowering phenotype of ftip1-1, indicating that NnFTIP1 has a conserved function as FTIP1. NnFTIP1 and NnFT1 share similar tissue expression patterns and subcellular localization. NnFTIP1 and NnFT1 interact both in vitro and in vivo. In addition, NnFTIP1 affects NnFT1 transport from leaves to the SAM. Furthermore, we found that NnUOF8, a MYB-like transcription factor, directly regulates the expression of NnFTIP1. Our results suggest that the functions of FTIP1 and FT are conserved during evolution in flowering plants.
在高等植物中，花信号主要在拟南芥及其同源物中被收集并转导到开花时间基因（FT）。FT 从叶片到茎尖分生组织（SAM）的移动部分由 FT 相互作用蛋白 1（FTIP1）介导。虽然 OsFTIP1 在水稻和 DOFTIP1 在兰花中的 FT 运输功能也已经被研究过，但在具有高经济和文化价值的园艺植物荷花（Nelumbo nucifera Gaertn.）中，尚未分离出 FTIP1 同源物，并且 NnFT1 运输的机制也尚未探索。在这里，我们揭示了 NnFTIP1 介导了拟南芥异位转基因系中 NnFT1 的运输。在 ftip1-1 背景下过表达 NnFTIP1 挽救了 ftip1-1 的晚花表型，表明 NnFTIP1 具有作为 FTIP1 的保守功能。NnFTIP1 和 NnFT1 具有相似的组织表达模式和亚细胞定位。NnFTIP1 和 NnFT1 在体外和体内相互作用。此外，NnFTIP1 影响 NnFT1 从叶片到 SAM 的运输。此外，我们发现 MYB 样转录因子 NnUOF8 直接调控 NnFTIP1 的表达。我们的结果表明，在开花植物的进化过程中，FTIP1 和 FT 的功能是保守的。
