Department of Neonatology, the Third Affiliated Hospital of Zhengzhou University, Zhengzhou 450052, Henan, China; Department of Academy of Medical Sciences of Zhengzhou University, Zhengzhou 450052, Henan, China.
Henan Key Laboratory of Child Brain Injury, the Third Affiliated Hospital of Zhengzhou University, Zhengzhou 450052, Henan, China.
Hum Immunol. 2021 Jan;82(1):54-59. doi: 10.1016/j.humimm.2020.11.002. Epub 2020 Dec 5.
As the survival rate of premature infants increases, the incidence of bronchopulmonary dysplasia (BPD), a chronic complication of premature infants, is also higher than before. The pathogenesis of BPD is complicated, and immune imbalance and inflammatory response may play important roles in it.
To investigate the correlation between lymphocyte subsets in peripheral blood, especially γδ-T cells, and BPD of preterm infants.
The study was carried out with the peripheral blood of premature infants (GA < 32 weeks, BW < 1500 g), which were collected at 24 h or 3-4 weeks after birth. The infants were divided into non-BPD groups and BPD groups that were classified as mild or moderate and severe in preterm infants based on the magnitude of respiratory support at 28 days age and 36 weeks postmenstrual age. The γδ-T, CD3+, CD4+, CD8+ and total lymphocyte subsets in peripheral blood were detected by flow cytometry.
The percentages of T lymphocyte subsets in peripheral blood were not different between BPD and non-BPD within 24 h after birth. And no significant difference was found in T lymphocyte subsets among neonates with BPD of different severities. However, the infants who developed BPD had a significant increase in γδ-T cells compared to non-BPD ones within 3-4 weeks after birth.
It seems that γδ-T cells in peripheral blood are correlated with BPD. However, the causality of BPD and various lymphocytes remains unclear, which need to be further studied.
随着早产儿存活率的提高，支气管肺发育不良（BPD）这一早产儿的慢性并发症的发生率也高于以往。BPD 的发病机制复杂，免疫失衡和炎症反应可能在其中发挥重要作用。
探讨早产儿外周血淋巴细胞亚群，尤其是 γδ-T 细胞与 BPD 的相关性。
本研究纳入了胎龄（GA）＜32 周、出生体重（BW）＜1500 g 的早产儿外周血，于出生后 24 h 或 3-4 周采集。根据早产儿出生后 28 天和 36 周校正胎龄时的呼吸支持程度，将婴儿分为非 BPD 组和 BPD 组（分为轻度和中重度）。采用流式细胞术检测外周血 γδ-T、CD3+、CD4+、CD8+和总淋巴细胞亚群。
出生后 24 h 内，BPD 组与非 BPD 组外周血 T 淋巴细胞亚群比例无差异。BPD 严重程度不同的新生儿 T 淋巴细胞亚群之间也无显著差异。然而，与非 BPD 组相比，出生后 3-4 周发生 BPD 的婴儿外周血 γδ-T 细胞显著增加。
似乎外周血中的 γδ-T 细胞与 BPD 相关。然而，BPD 与各种淋巴细胞之间的因果关系尚不清楚，需要进一步研究。
