Pshennikova E S, Kolot M N, Khmel' I A
Mol Biol (Mosk). 1987 Nov-Dec;21(6):1560-5.
The organization of the genes involved in colicin D synthesis was studied. These are colicin, immunity and lysis genes. The nucleotide sequence of the immunity gene, its structural and regulatory regions were determined. This gene was shown to be located next to the colicin gene on the same strand and followed by the lysis gene. When colicin synthesis is induced with mitomycin C the immunity gene is transcribed from the general SOS-dependent promotor as a part of the colicin operon. However it has its own SOS-independent promotor in normal growth conditions. A high homology in amino acid sequences of Co1D lysis protein and that of Co1E1, Co1E2, Co1E3, Co1DF13, Co1A was revealed. A detailed scheme of Co1D-CA23 colicin operon structural organization is suggested.
对参与大肠杆菌素D合成的基因组织进行了研究。这些基因包括大肠杆菌素基因、免疫基因和裂解基因。确定了免疫基因的核苷酸序列及其结构和调控区域。该基因位于与大肠杆菌素基因同一条链上且紧挨着大肠杆菌素基因，后面跟着裂解基因。当用丝裂霉素C诱导大肠杆菌素合成时，免疫基因作为大肠杆菌素操纵子的一部分从一般的SOS依赖启动子转录。然而，在正常生长条件下它有自己的SOS非依赖启动子。揭示了Co1D裂解蛋白与Co1E1、Co1E2、Co1E3、Co1DF13、Co1A的氨基酸序列具有高度同源性。提出了Co1D-CA23大肠杆菌素操纵子结构组织的详细示意图。
