Laboratorio de Biotecnología Molecular. Instituto de Biotecnología Misiones. CONICET. Facultad de Ciencias Exactas, Químicas y Naturales. Universidad Nacional de Misiones, CP3300, Posadas, Misiones, Argentina.
Laboratorio de Biotecnología Molecular. Instituto de Biotecnología Misiones. CONICET. Facultad de Ciencias Exactas, Químicas y Naturales. Universidad Nacional de Misiones, CP3300, Posadas, Misiones, Argentina.
Chemosphere. 2021 Feb;265:129093. doi: 10.1016/j.chemosphere.2020.129093. Epub 2020 Nov 24.
White-rot fungi are well known bioremediation agents capable of removing recalcitrant xenobiotics. However, the molecular mechanism involved in this process is not well understood. The aim of the present study was to compare the proteomic profiles of Pleurotus pulmonarius LBM 105 in presence and absence of a mixture of polychlorinated biphenyls. Cultures of the fungus were spiked with a mixture of Aroclors and cultivated for 28 days. This strain achieved a peak of PCBs-removal of 65.50 ± 8.09% after 21 days. The ecotoxicological assays showed a toxicity reduction of 46.47%. Based on these findings, a proteomic study was carried out and it was proven that the oxidative metabolism was highly affected. Two proteins that have a function at the transcriptional level and related to the oxidative metabolism, the glyceraldehyde-3-phosphate dehydrogenase and the basal transcription factor 3, presented an increase in their quantity in PCBs presence. Several oxidases and reductases were highly induced, presenting the short chain reductases, aldo/keto reductases, laccases and versatile peroxidases as the enzymes with the most notorious changes. These results indicate a complex response of the fungal metabolism towards these pollutants, which includes a transcriptional response to the oxidative stress and a modification of the intra- and extra-cellular enzymatic profile.
白腐真菌是众所周知的生物修复剂，能够去除难降解的外来化合物。然而，这一过程中的分子机制尚不清楚。本研究的目的是比较在存在和不存在多氯联苯混合物的情况下，肺形侧耳 LBM 105 的蛋白质组图谱。将真菌的培养物与 Aroclors 混合物混合并培养 28 天。该菌株在 21 天后达到 PCB 去除率的峰值 65.50±8.09%。生态毒理学试验表明毒性降低了 46.47%。基于这些发现，进行了蛋白质组学研究，证明氧化代谢受到了很大影响。两种具有转录水平功能且与氧化代谢有关的蛋白质，甘油醛-3-磷酸脱氢酶和基本转录因子 3，在 PCB 存在时其数量增加。几种氧化酶和还原酶被高度诱导，其中短链还原酶、醛/酮还原酶、漆酶和多功能过氧化物酶作为变化最显著的酶。这些结果表明，真菌代谢对这些污染物的复杂反应，包括对氧化应激的转录反应和细胞内外酶谱的修饰。
