Laboratorio de Biotecnología Molecular, Instituto de Biotecnología Misiones,, CONICET, Facultad de Ciencias Exactas Químicas y Naturales, Universidad Nacional de Misiones, Posadas, Misiones, CP3300, Argentina.
J Appl Microbiol. 2021 Oct;131(4):1775-1786. doi: 10.1111/jam.15073. Epub 2021 Mar 25.
To evaluate the mycoremediation of polychlorinated biphenyls (PCBs) by either single cultures or binary consortia of Pleurotus pulmonarius LBM 105 and Trametes sanguinea LBM 023.
PCBs tolerance, removal capacity, toxicity reduction and ligninolytic enzyme expression were assessed when growing single culture and binary consortium of fungus in 217 mg l of a technical mixture of Aroclor 1242, 1254 and 1260 in transformer oil. A decrease in tolerance and variation in ligninolytic enzyme secretion were observed in PCB-amended solid media. Pleurotus pulmonarius LBM 105 mono-culture was able to remove up to 95·4% of PCBs, whereas binary consortium and T. sanguinea LBM 023 could biodegrade about 55% after 24 days. Significant detoxification levels were detected in all treatments by biosorption mechanism.
Pleurotus pulmonarius LBM 105 in single culture had the best performance regarding PCBs biodegradation and toxicity reduction. Ligninolytic enzyme secretion changed in co-culture.
The evaluation of PCBs bioremediation effectiveness of basidiomycetes consortium in terms of PCB removal, toxicity and ligninolytic enzyme production to unravel the differences between using individual cultures or consortium has not been reported. The results from this study enable the selection of P. pulmonarius LBM 105 mono-culture to bioremediate PCBs as it showed higher efficiency compared to binary consortium with T. sanguinea LBM 023 for potential decontamination of PCB-contaminated transformer oil.
评估肺侧耳 LBM 105 和红栓菌 LBM 023 单一培养物或二元共生体对多氯联苯（PCBs）的真菌修复作用。
在变压器油中 217mg/L 的 Aroclor 1242、1254 和 1260 技术混合物中，评估单一真菌培养物和二元共生体生长时的 PCB 耐受性、去除能力、毒性降低和木质素降解酶表达。在添加 PCB 的固体培养基中，观察到耐受性降低和木质素降解酶分泌的变化。肺侧耳 LBM 105 单培养物能够去除高达 95.4%的 PCBs，而二元共生体和红栓菌 LBM 023 在 24 天后能够生物降解约 55%。所有处理均通过生物吸附机制检测到显著的解毒水平。
在单培养物中，肺侧耳 LBM 105 在 PCB 生物降解和毒性降低方面表现最佳。共培养物中木质素降解酶的分泌发生了变化。
尚未有报道评估担子菌共生体在 PCB 去除、毒性和木质素降解酶产生方面对多氯联苯生物修复效果的评价，以揭示使用单一培养物或共生体的差异。本研究结果使我们能够选择肺侧耳 LBM 105 进行单培养物生物修复 PCBs，因为与红栓菌 LBM 023 组成的二元共生体相比，它显示出更高的效率，可用于潜在净化 PCB 污染的变压器油。
