Departamento Química Analítica, Facultad Química, Universidad Sevilla, C/Prof. García González, S/n, 41012, Sevilla, Spain; Université Côte D'Azur, CNRS, Institut de Chimie de Nice, 28 Avenue Valrose, 06108, Nice, CEDEX 2, France.
Université Côte D'Azur, CNRS, Institut de Chimie de Nice, 28 Avenue Valrose, 06108, Nice, CEDEX 2, France.
Chemosphere. 2021 Mar;266:128995. doi: 10.1016/j.chemosphere.2020.128995. Epub 2020 Nov 19.
In this work, eight plants of Juncus sp. and ten of Salicornia europaea were used for an uptake assay of pharmaceuticals (flumequine, cirpofloxacin, enrofloxacin, carbamazepine, diclofenac and ibuprofen) by irrigation at three concentration levels: 10 ng mL (low level); 700 ng mL (medium level) and 10 μg mL (high level). Two plants irrigated with pharmaceutical-free water were set up as controls. For each level, two plants were watered every day with 50 mL (Juncus sp.) and every two days with 20 mL (Salicornia europaea) of aqueous solutions containing all the analytes at the described concentrations. Plants irrigated at 10 μg mL were significantly the most affected, whereas the rest of the plants remained, in general, largely displayed no apparent physiological effects throughout the 30 days (Juncus sp.) and 21 days (Salicornia europaea) assays. Leaves and stems were cut every seven days and roots were collected at the end of the assay. The samples were lyophilized, submitted to a microwave assisted extraction using 5 mL of acetonitrile:water mixture (1:1, v/v) and they were analyzed (in triplicate) in a liquid chromatography-quadrupole time of flight mass spectrometry instrument. Most of the analytes were quantified in many of the samples corresponding to the three exposure levels with the highest concentrations obtained at high exposure levels. Ibuprofen was not detected in any sample and enrofloxacin, ciprofloxacin and diclofenac were not detected in the samples from Salicornia europaea.
在这项工作中，使用了 8 株灯心草属植物和 10 株滨藜属植物进行了药物摄取分析，灌溉药物有氟甲喹、环丙沙星、恩诺沙星、卡马西平、双氯芬酸和布洛芬，浓度水平分别为 10ng mL（低水平）、700ng mL（中水平）和 10μg mL（高水平）。设置了用不含药物的水灌溉的两株植物作为对照。对于每个水平，每天用 50mL（灯心草属植物）和每两天用 20mL（滨藜属植物）的含所有分析物的水溶液灌溉两株植物，溶液浓度如上所述。用 10μg mL 灌溉的植物受到的影响最大，而其余的植物在 30 天（灯心草属植物）和 21 天（滨藜属植物）的试验中，总体上仍基本没有表现出明显的生理效应。每隔七天采集叶片和茎，在试验结束时采集根。将样品冻干，用 5mL 乙腈：水混合液（1:1，v/v）进行微波辅助提取，然后在液相色谱-四极杆飞行时间质谱仪中进行分析（一式三份）。在对应三个暴露水平的许多样品中都能定量大多数分析物，并且在高暴露水平下获得了最高浓度。在任何样品中都未检测到布洛芬，而在滨藜属植物的样品中也未检测到恩诺沙星、环丙沙星和双氯芬酸。
