长链非编码 RNA 在卵巢癌中的生物学作用。
The Biological Role of the Long Non-coding RNA in Ovarian Carcinoma.
机构信息
Institute of Drug Research, School of Pharmacy, Faculty of Medicine, The Hebrew University of Jerusalem, Jerusalem, Israel.
Department of Pathology, Oslo University Hospital, Norwegian Radium Hospital, Oslo, Norway;
出版信息
Anticancer Res. 2020 Dec;40(12):6677-6684. doi: 10.21873/anticanres.14691.
AIM
To analyze the biological role of the long non-coding RNA LINK-A.
MATERIALS AND METHODS
An 850-bp segment from the second exon of LINK-A was removed using the CRISPR/Cas9 system in OVCA433 ovarian serous carcinoma cells. Spheroid formation, migration, invasion, proliferation, matrix metalloproteinase (MMP) activity and expression of cell-signaling proteins were assessed in vitro.
RESULTS
OVCA433 cells with LINK-A deletion were more invasive (p=0.0008) but had reduced migration and MMP9 secretion compared to controls (p=0.003 and p=0.005, respectively). LINK-A deletion did not affect proliferation but induced phosphorylation of extracellular signal-regulated kinase (10-fold; p=0.005). LINK-A knock out additionally reduced spheroid formation.
CONCLUSION
Added to our previous data from analysis of clinical specimens, LINK-A is likely to be a tumor suppressor.
目的
分析长链非编码 RNA LINK-A 的生物学功能。
材料和方法
利用 CRISPR/Cas9 系统从 LINK-A 的第二个外显子中切除 850bp 片段，在卵巢浆液性癌细胞系 OVCA433 中进行研究。体外评估球体形成、迁移、侵袭、增殖、基质金属蛋白酶（MMP）活性和细胞信号蛋白的表达。
结果
与对照组相比，LINK-A 缺失的 OVCA433 细胞侵袭性更强（p=0.0008），但迁移和 MMP9 分泌减少（p=0.003 和 p=0.005）。LINK-A 缺失不影响增殖，但诱导细胞外信号调节激酶磷酸化（增加 10 倍；p=0.005）。LINK-A 敲除还减少了球体形成。
结论
结合我们之前对临床标本的分析数据，LINK-A 可能是一种肿瘤抑制因子。
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