INCLIVA, Research Institute Valencia, Hospital Clínico Universitario de Valencia, Valencia, Spain.
Department of Physiology, University of Valencia, Valencia, Spain.
Anticancer Res. 2020 Dec;40(12):6853-6861. doi: 10.21873/anticanres.14707.
BACKGROUND/AIM: In chronic liver disease, various immune cell subsets exert pro or anti-tumour effects by releasing reactive oxygen and nitrogen species (ROS, RNS). Here, we evaluated the oxidative and nitrosative pattern in peripheral blood leukocyte subpopulations of early hepatocellular carcinoma (HCC) patients compared with HCC-free cirrhotic patients.
Venous blood samples from 18 HCC-free cirrhotic patients and 17 early stage HCC patients were collected to determine ROS, RNS and reduced glutathione levels in isolated leukocytes analyzed by flow cytometry.
Intracellular levels of ROS and glutathione were higher in lymphocytes, monocytes, and neutrophils from HCC patients as well as mitochondrial superoxide in neutrophils and monocytes whereas intracellular levels of nitric oxide were lower in lymphocytes, monocytes, and neutrophils.
Early HCC alters intracellular levels of ROS and RNS of some circulating leukocytes subsets. This finding may represent a potential area of interest concerning the development of new treatments and prognostic markers.
背景/目的：在慢性肝脏疾病中，各种免疫细胞亚群通过释放活性氧和氮物种（ROS、RNS）发挥抗肿瘤或促肿瘤作用。在这里，我们评估了早期肝细胞癌（HCC）患者与无 HCC 肝硬化患者外周血白细胞亚群中的氧化和硝化模式。
收集了 18 名无 HCC 肝硬化患者和 17 名早期 HCC 患者的静脉血样本，通过流式细胞术分析分离的白细胞中 ROS、RNS 和还原型谷胱甘肽的水平。
HCC 患者的淋巴细胞、单核细胞和中性粒细胞中 ROS 和谷胱甘肽的细胞内水平以及中性粒细胞和单核细胞中的线粒体超氧阴离子更高，而淋巴细胞、单核细胞和中性粒细胞中的一氧化氮细胞内水平较低。
早期 HCC 改变了一些循环白细胞亚群的 ROS 和 RNS 的细胞内水平。这一发现可能代表了开发新治疗方法和预后标志物的潜在研究领域。
