Department of Chinese Medicine, Ditmanson Medical Foundation Chiayi Christian Hospital, Chiayi, Taiwan, R.O.C.
Department of Sports Management, Chia Nan University of Pharmacy & Science, Tainan City, Taiwan, R.O.C.
Anticancer Res. 2020 Dec;40(12):6907-6914. doi: 10.21873/anticanres.14714.
BACKGROUND/AIM: Hepatocellular carcinoma (HCC) is the most common primary malignancy of the liver. Resistance to chemotherapy and side-effects remain a challenge for treating advanced and recurrent HCC. Therefore, there is an emerging need to develop new drugs to treat HCC.
We evaluated the anti-growth activity of flavopereirine in HepG2 and Huh7 HCC cell lines. Cell viability, cell-cycle profile, apoptosis, and autophagy-related protein expressions were analysed after flavopereirine treatment.
Flavopereirine treatment induced G/G cell-cycle arrest, with an increase of sub-G cells detected at the higher concentration and longer exposure to flavopereirine in HCC cells. Intrinsic and extrinsic pathways were involved in flavopereirine-induced apoptosis, as demonstrated by an increase of cleaved caspase 8 and 9 by western blotting. An alteration of autophagy-related protein expression was also found after flavopereirine treatment.
Taken together, the current results indicate that flavopereirine exhibits good anticancer activity in HCC cells.
背景/目的：肝细胞癌（HCC）是肝脏最常见的原发性恶性肿瘤。化疗耐药和副作用仍然是治疗晚期和复发性 HCC 的挑战。因此，迫切需要开发新的药物来治疗 HCC。
我们评估了 flavopereirine 在 HepG2 和 Huh7 HCC 细胞系中的抗生长活性。 flavopereirine 处理后，分析细胞活力、细胞周期谱、细胞凋亡和自噬相关蛋白表达。
flavopereirine 处理诱导 G/G 细胞周期阻滞，在较高浓度和更长时间暴露于 flavopereirine 时，检测到亚 G 期细胞增加。Western blot 分析表明，flavopereirine 诱导的细胞凋亡涉及内在和外在途径。 flavopereirine 处理后还发现自噬相关蛋白表达的改变。
综上所述，目前的结果表明 flavopereirine 在 HCC 细胞中表现出良好的抗癌活性。
