College of Chemistry and Molecular Engineering, Synthetic and Functional Biomolecules Center, Beijing National Laboratory for Molecular Sciences, Key Laboratory of Bioorganic Chemistry and Molecular Engineering, Ministry of Education, Peking University, Beijing, 100871, P. R. China.
Peking-Tsinghua Center for Life Sciences, Peking University, Beijing, 100871, P. R. China.
Chembiochem. 2021 Apr 16;22(8):1357-1367. doi: 10.1002/cbic.202000747. Epub 2021 Jan 26.
Nature uses a diverse array of protein post-translational modifications (PTMs) to regulate protein structure, activity, localization, and function. Among them, protein 4'-phosphopantetheinylation derived from coenzyme A (CoA) is an essential PTM for the biosynthesis of fatty acids, polyketides, and nonribosomal peptides in prokaryotes and eukaryotes. To explore its functions, various chemical probes mimicking the natural structure of 4'-phosphopantetheinylation have been developed. In this minireview, we summarize these chemical probes and describe their applications in direct and metabolic labeling of proteins in bacterial and mammalian cells.
自然界利用多种蛋白质翻译后修饰（PTMs）来调节蛋白质的结构、活性、定位和功能。其中，来源于辅酶 A（CoA）的蛋白质 4'-磷酸泛酰巯基乙胺是原核生物和真核生物脂肪酸、聚酮和非核糖体肽生物合成所必需的 PTM。为了探索其功能，已经开发了各种模拟 4'-磷酸泛酰巯基乙胺天然结构的化学探针。在这篇综述中，我们总结了这些化学探针，并描述了它们在细菌和哺乳动物细胞中蛋白质的直接和代谢标记中的应用。
