School of Engineering, Macquarie University, Sydney, New South Wales 2109, Australia.
Suqian University, Suqian, 223800, China.
Anal Chem. 2021 Jan 26;93(3):1586-1595. doi: 10.1021/acs.analchem.0c03990. Epub 2020 Dec 8.
Yeast () is one of the most attractive microbial species used for industrial production of value-added products and is an important model organism to understand the biology of the eukaryotic cells and humans. has different shapes, such as spherical singlets, budded doublets, and clusters, corresponding to phases of the cell cycle, genetic, and environmental factors. The ability to obtain high-purity populations of uniform-shaped cells is of significant importance for a wide range of applications in basic biological research and industrial processes. In this work, we demonstrate shape-based separation and enrichment of using a coflow of viscoelastic and Newtonian fluids in a straight rectangular microchannel. Due to the combined effects of lift inertial and elastic forces, this label-free and continuous separation arises from shape-dependent migration of cells from the Newtonian to the non-Newtonian viscoelastic fluid. The lateral position of cells with varying morphologies is found to be dependent on cell major axis. We also investigate the effects of sheath and sample flow rate, poly(ethylene oxide) (PEO) concentration and channel length on the performance of the viscoelastic microfluidic device for enrichment and separation by shape. Moreover, the separation efficiency, cell extraction yield, and cell viability after sorting operations are studied.
酵母（Yeast）是最具吸引力的微生物物种之一，用于工业生产增值产品，是了解真核细胞和人类生物学的重要模式生物。酵母具有不同的形状，如球形单体、芽生的双联体和簇，对应于细胞周期、遗传和环境因素的不同阶段。获得具有均匀形状的高纯度酵母种群对于基础生物学研究和工业过程的广泛应用具有重要意义。在这项工作中，我们展示了使用粘弹性和牛顿流体共流在直矩形微通道中基于形状的分离和浓缩酵母。由于升力惯性和弹性力的综合作用，这种无标记和连续的分离来自于细胞从牛顿流体到非牛顿粘弹性流体的形状依赖性迁移。具有不同形态的酵母细胞的横向位置被发现取决于细胞的长轴。我们还研究了鞘液和样品流速、聚（环氧乙烷）（PEO）浓度和通道长度对通过形状进行酵母浓缩和分离的粘弹性微流控装置性能的影响。此外，还研究了分选操作后的分离效率、细胞提取产量和细胞活力。
