Department of Plastic and Reconstructive Surgery, CMRI, School of Medicine, Kyungpook National University, Kyungpook National University Hospital, Daegu, Republic of Korea.
Department of Nuclear Medicine, School of Medicine, Kyungpook National University, Daegu, Republic of Korea.
Stem Cells. 2021 Mar;39(3):266-279. doi: 10.1002/stem.3310. Epub 2020 Dec 31.
Extracellular vesicles (EVs) have been exhibited as promising candidates for delivering endogenous therapeutic cargos for regenerative therapies. Fibroblasts could be candidate source cells for EVs, to investigate their therapeutic effects in wound healing. Here we demonstrated the isolation and characterization of fibroblast-derived (L929 cell line) EVs (L929-EVs). Furthermore, L929-EVs treatment showed pro-wound healing effects in vitro by enhancing proliferation, migration, and scarless wound healing related genes in fibroblast cells. L929-EVs treatment also enhanced the migration and tube formation of endothelial cells. The combination of L929-EVs with fibrin glue accelerated wound healing in the mouse skin wound model by enhancing collagen formation, collagen maturation, and blood vessels in the wounded skin. The role of fibroblast-derived EVs in wound healing could be an important phenomenon, and fibroblast-derived EVs could be harnessed for wound healing therapies.
细胞外囊泡 (EVs) 已被证明是递送内源性治疗性 cargo 用于再生治疗的有前途的候选物。成纤维细胞可以作为 EVs 的候选来源细胞，以研究其在伤口愈合中的治疗效果。在这里，我们展示了成纤维细胞衍生的 (L929 细胞系) EVs (L929-EVs) 的分离和表征。此外，L929-EVs 处理通过增强成纤维细胞中增殖、迁移和无瘢痕愈合相关基因的表达，在体外表现出促伤口愈合作用。L929-EVs 处理还增强了内皮细胞的迁移和管状形成。L929-EVs 与纤维蛋白胶的联合应用通过增强伤口皮肤中的胶原形成、胶原成熟和血管，加速了小鼠皮肤伤口模型中的伤口愈合。成纤维细胞衍生的 EVs 在伤口愈合中的作用可能是一个重要现象，成纤维细胞衍生的 EVs 可用于伤口愈合治疗。
