College of Life Science, Northwest Normal University, Lanzhou, 730070, PR China.
College of Life Science, Northwest Normal University, Lanzhou, 730070, PR China.
Cryobiology. 2021 Feb;98:119-126. doi: 10.1016/j.cryobiol.2020.11.016. Epub 2020 Dec 5.
Chorispora bungeana (C. bungeana) is a rare alpine subnival species that is highly tolerant to low temperature stress. Phospholipase D (PLD) is a key enzyme involved in membrane phospholipid catabolism during plant growth and the stress response. In this study, one member of CbPLD gene family, CbPLDδ, was cloned from C. bungeana and was introduced into tobacco. This gene encodes an 864-amino acid protein with two catalytic HxKxxxxD motifs which are essential for phospholipase D activity. After the CbPLDδ gene is fused with the vector containing the GFP tag, subcellular localization showed that CbPLDδ was predominately located in the cell membrane. RT-qPCR and histochemical GUS assays showed that CbPLDδ gene was induced by low temperature and expressed predominantly in leaf and root. Compared with wild-type tobacco, CbPLDδ transgenic tobacco showed higher activities of antioxidant enzymes, and lower levels of malonidiadehyde and electrolyte leakage under low temperature stress. These results reflected that CbPLDδ is involved in the response to low temperature stress, and has the potential to improve the low temperature tolerance of plants.
沙芥（Chorispora bungeana）是一种耐低温的高山亚冰雪区特有种，磷脂酶 D（PLD）是植物生长和逆境响应过程中参与膜磷脂分解代谢的关键酶。本研究从沙芥中克隆得到了一个 PLD 基因家族成员 CbPLDδ，并将其转入烟草中。该基因编码一个 864 个氨基酸的蛋白，具有两个催化 HxKxxxxD 基序，是磷脂酶 D 活性所必需的。将 CbPLDδ 基因与含有 GFP 标签的载体融合后，亚细胞定位显示 CbPLDδ 主要定位于细胞膜上。RT-qPCR 和组织化学 GUS 分析表明，CbPLDδ 基因受低温诱导，主要在叶片和根中表达。与野生型烟草相比，CbPLDδ 转基因烟草在低温胁迫下表现出更高的抗氧化酶活性，丙二醛和电解质泄漏水平更低。这些结果表明 CbPLDδ 参与了低温胁迫响应，并具有提高植物耐低温性的潜力。
