Steiner Evyatar, Israeli Alon, Gupta Rupali, Shwartz Ido, Nir Ido, Leibman-Markus Meirav, Tal Lior, Farber Mika, Amsalem Ziva, Ori Naomi, Müller Bruno, Bar Maya
Institute of Plant Sciences and Genetics in Agriculture, Hebrew University of Jerusalem, 7610001, Rehovot, Israel.
Department of Plant Pathology and Weed Research, Plant Protection Institute, Agricultural Research Organization, The Volcani Center, 7505101, Rishon LeZion, Israel.
Plant Methods. 2020 Nov 16;16(1):152. doi: 10.1186/s13007-020-00694-2.
Hormones are crucial to plant life and development. Being able to follow the plants hormonal response to various stimuli and throughout developmental processes is an important and increasingly widespread tool. The phytohormone cytokinin (CK) has crucial roles in the regulation of plant growth and development.
Here we describe a version of the CK sensor Two Component signaling Sensor (TCS), referred to as TCSv2. TCSv2 has a different arrangement of binding motifs when compared to previous TCS versions, resulting in increased sensitivity in some examined tissues. Here, we examine the CK responsiveness and distribution pattern of TCSv2 in arabidopsis and tomato.
The increased sensitivity and reported expression pattern of TCSv2 make it an ideal TCS version to study CK response in particular hosts, such as tomato, and particular tissues, such as leaves and flowers.
激素对植物的生命和发育至关重要。能够追踪植物对各种刺激以及整个发育过程中的激素反应是一种重要且日益广泛应用的工具。植物激素细胞分裂素(CK)在植物生长和发育的调控中具有关键作用。
在此,我们描述了一种细胞分裂素传感器双组分信号传感器(TCS)的版本,称为TCSv2。与先前的TCS版本相比,TCSv2具有不同的结合基序排列,导致在一些检测的组织中敏感性增加。在此,我们研究了TCSv2在拟南芥和番茄中的细胞分裂素反应性和分布模式。
TCSv2增加的敏感性和报道的表达模式使其成为研究特定宿主(如番茄)和特定组织(如叶片和花朵)中细胞分裂素反应的理想TCS版本。