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初级多步磷酸化级联激活包含细胞分裂素和非生物胁迫响应：拟南芥 A 型反应调节蛋白的比较分析得出的见解。

Primary multistep phosphorelay activation comprises both cytokinin and abiotic stress responses: insights from comparative analysis of Brassica type-A response regulators.

机构信息

CEITEC - Central European Institute of Technology, Masaryk University, Kamenice 5/A2, 625 00 Brno, Czech Republic.

National Centre for Biomolecular Research, Faculty of Science, Masaryk University, Kamenice 5/A2, 625 00 Brno, Czech Republic.

出版信息

J Exp Bot. 2024 Oct 30;75(20):6346-6368. doi: 10.1093/jxb/erae335.

DOI:10.1093/jxb/erae335
PMID:39171371
原文链接:https://pmc.ncbi.nlm.nih.gov/articles/PMC11523033/
Abstract

Multistep phosphorelay (MSP) signaling integrates hormonal and environmental signals to control both plant development and adaptive responses. Type-A RESPONSE REGULATOR (RRA) genes, the downstream members of the MSP cascade and cytokinin primary response genes, are thought to mediate primarily the negative feedback regulation of (cytokinin-induced) MSP signaling. However, transcriptional data also suggest the involvement of RRA genes in stress-related responses. By employing evolutionary conservation with the well-characterized Arabidopsis thaliana RRA genes, we identified five and 38 novel putative RRA genes in Brassica oleracea and Brassica napus, respectively. Our phylogenetic analysis suggests the existence of gene-specific selective pressure, maintaining the homologs of ARR3, ARR6, and ARR16 as singletons during the evolution of Brassicaceae. We categorized RRA genes based on the kinetics of their cytokinin-mediated up-regulation and observed both similarities and specificities in this type of response across Brassicaceae species. Using bioinformatic analysis and experimental data demonstrating the cytokinin and abiotic stress responsiveness of the A. thaliana-derived TCSv2 reporter, we unveil the mechanistic conservation of cytokinin- and stress-mediated up-regulation of RRA genes in B. rapa and B. napus. Notably, we identify partial cytokinin dependency of cold stress-induced RRA transcription, thus further demonstrating the role of cytokinin signaling in crop adaptive responses.

摘要

多步磷酸接力（MSP）信号整合了激素和环境信号，以控制植物的发育和适应性反应。A型应答调节因子（RRA）基因是 MSP 级联的下游成员和细胞分裂素的主要反应基因，被认为主要介导（细胞分裂素诱导的）MSP 信号的负反馈调节。然而，转录数据也表明 RRA 基因参与了与应激相关的反应。通过利用与拟南芥中已充分研究的 RRA 基因的进化保守性，我们分别在甘蓝型油菜和甘蓝型油菜中鉴定出了 5 个和 38 个新的假定 RRA 基因。我们的系统发育分析表明，存在基因特异性的选择压力，在芸薹科植物的进化过程中，将 ARR3、ARR6 和 ARR16 的同源物维持为单体。我们根据其细胞分裂素介导的上调的动力学对 RRA 基因进行了分类，并在芸薹科物种中观察到了这种反应的相似性和特异性。通过生物信息学分析和实验数据证明了来源于拟南芥的 TCSv2 报告基因对细胞分裂素和非生物胁迫的响应，我们揭示了 RRA 基因在油菜和油菜中细胞分裂素和应激介导的上调的机制保守性。值得注意的是，我们确定了冷胁迫诱导的 RRA 转录对细胞分裂素的部分依赖性，从而进一步证明了细胞分裂素信号在作物适应性反应中的作用。

https://cdn.ncbi.nlm.nih.gov/pmc/blobs/26b4/11523033/1c9f0db65ca9/erae335_fig11.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/26b4/11523033/0257bc3a6d00/erae335_fig1.jpg
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https://cdn.ncbi.nlm.nih.gov/pmc/blobs/26b4/11523033/423b9f5c35b3/erae335_fig4.jpg
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https://cdn.ncbi.nlm.nih.gov/pmc/blobs/26b4/11523033/a8746b7ff8f3/erae335_fig9.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/26b4/11523033/6381c8282921/erae335_fig10.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/26b4/11523033/1c9f0db65ca9/erae335_fig11.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/26b4/11523033/0257bc3a6d00/erae335_fig1.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/26b4/11523033/4592ad6ebf55/erae335_fig2.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/26b4/11523033/0e7905f7b6a3/erae335_fig3.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/26b4/11523033/423b9f5c35b3/erae335_fig4.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/26b4/11523033/e79c5769f641/erae335_fig5.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/26b4/11523033/fc27c348735d/erae335_fig6.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/26b4/11523033/597f20a6ad84/erae335_fig7.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/26b4/11523033/bf574fc500b1/erae335_fig8.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/26b4/11523033/a8746b7ff8f3/erae335_fig9.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/26b4/11523033/6381c8282921/erae335_fig10.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/26b4/11523033/1c9f0db65ca9/erae335_fig11.jpg

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2
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3
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4
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Genes (Basel). 2022 Aug 15;13(8):1449. doi: 10.3390/genes13081449.
5
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8
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