Lee Ju Young, Kim Sang Eun, Lee Hoon Taek, Hwang Jeong Ho
Animal Model Research Group, Korea Institute of Toxicology, 30, Baekhak 1-gil, Jeongeup-si, Jeollabuk-do, 143-701, South Korea.
Division of Human and Environmental Toxicology, University of Science & Technology, Daejeon, South Korea.
Lab Anim Res. 2020 Dec 1;36(1):44. doi: 10.1186/s42826-020-00077-7.
Recently, minipig has been considered as an animal model that is appropriate for human disease model to study toxicology, pharmacology, and xenotransplantation. Nevertheless, minipigs are bred in various environment according to their use. Here, we suggest that minipigs used for research should be bred in well-controlled facility, comparing immune status of pigs raised in different breeding environment. DNA microarray was performed with ear skin and placenta of Landrace domestic pigs (DPs) and Minnesota germ-free minipigs (GPs). Their immune transcriptome was analyzed by gene ontology (GO) annotation database, based on criteria of |log fold change| ≥1 with P ≤ 0.05. As a result, we found that immune related genes in the ear skin of DPs were highly activated, compared to GPs. On the other hand, no significant s were found in the placenta. Quantitative real-time PCR (qRT-PCR) was performed in five candidate immune genes. Their fold changes were consistent with the results from DNA microarray (P ≤ 0.05). In conclusion, we experimentally proved that porcine immune system was affected by different breeding environment, suggesting the importance of controlling microbes in animal room for the qualified research.
最近，小型猪已被视为一种适合用于人类疾病模型以研究毒理学、药理学和异种移植的动物模型。然而，小型猪根据其用途在不同环境中饲养。在此，我们建议用于研究的小型猪应在严格控制的设施中饲养，并比较在不同饲养环境中饲养的猪的免疫状态。对长白家猪（DPs）和明尼苏达无菌小型猪（GPs）的耳部皮肤和胎盘进行了DNA微阵列分析。基于|log倍数变化|≥1且P≤0.05的标准，通过基因本体论（GO）注释数据库对它们的免疫转录组进行了分析。结果，我们发现与GPs相比，DPs耳部皮肤中的免疫相关基因高度活化。另一方面，在胎盘中未发现显著差异。对五个候选免疫基因进行了定量实时PCR（qRT-PCR）。它们的倍数变化与DNA微阵列的结果一致（P≤0.05）。总之，我们通过实验证明了猪的免疫系统受不同饲养环境的影响，这表明在动物房中控制微生物对于合格研究的重要性。
