Research and External Innovation Department, Sanofi Pasteur, 69280 Marcy-l'Étoile, France.
Research and External Innovation Department, Sanofi Pasteur, 69280 Marcy-l'Étoile, France
Immunohorizons. 2020 Dec 8;4(12):762-773. doi: 10.4049/immunohorizons.2000094.
Lung tissue resident memory (T) T cells can provide rapid and effective protective immunity against respiratory pathogens such as We assessed an outbred CD1 mouse model and i.m. immunization to study vaccine-induced immune memory, using pertussis vaccines as an example. The phenotypes of cells from the lungs of CD1 mice that had been primed with either i.m. whole-cell (wP), acellular (aP) vaccines or buffer (unvaccinated) and challenged with were determined using flow cytometry and immunohistology. We observed a rapid and high increase of CD4T cells expressing T markers by flow cytometry, supported by immunohistology observations, in lungs from wP-immunized mice. Priming mice with wP vaccine induced a more potent CD4 response in lungs following challenge than priming with aP vaccine, although both were less potent than that observed in primoinfected mice. We also observed for the first time, to our knowledge, that CD8 and γδ T-like T cell responses were induced in lungs of wP-primed mice postinfection. This novel outbred CD1 mouse model with i.m. immunization that enabled us to study vaccine-induced -specific memory T cells in lungs could be useful for evaluating candidate parenteral vaccines against or others pulmonary pathogens.
肺组织驻留记忆 (T) T 细胞可以为呼吸道病原体（如 我们评估了一个远交 CD1 小鼠模型和肌肉内免疫接种，以研究疫苗诱导的免疫记忆，以百日咳疫苗为例。使用流式细胞术和免疫组织化学方法，确定了用肌肉内全细胞 (wP)、无细胞 (aP) 疫苗或缓冲液 (未接种疫苗) 预激的 CD1 小鼠肺部细胞的表型。我们观察到，通过流式细胞术观察到，与 aP 疫苗相比，wP 疫苗免疫接种的小鼠肺部快速且高度增加表达 T 标记的 CD4T 细胞，免疫组织化学观察结果也支持这一观点。尽管与初次感染的小鼠相比，这两种疫苗的作用都不如初次感染的小鼠，但在 挑战后，wP 疫苗预激小鼠的肺部 CD4 反应更为强烈。我们还首次观察到，在 wP 疫苗预激感染后的小鼠肺部诱导了 CD8 和 γδ T 样 T 细胞反应。这种新型的远交 CD1 小鼠肌肉内免疫接种模型使我们能够研究肺部疫苗诱导的 特异性记忆 T 细胞，可用于评估针对 或其他肺部病原体的候选肠道外疫苗。
