School of Biochemistry and Immunology, Trinity Biomedical Sciences Institute, Trinity College Dublin, Dublin, Ireland.
Children's Health Ireland, Dublin, Ireland.
J Infect Dis. 2024 Sep 23;230(3):e518-e523. doi: 10.1093/infdis/jiae034.
The objective was to determine if antigen-specific tissue-resident memory T (TRM) cells persist in respiratory tissues of adults immunized as children with whole-cell pertussis (wP) or acellular pertussis (aP) vaccines. Mononuclear cells from tonsil or nasal tissue cells were cultured with Bordetella pertussis antigens and TRM cells quantified by flow cytometry. Adults immunized with wP vaccines as children had significantly more interleukin 17A (IL-17A) and interferon-γ (IFN-γ)-producing TRM cells that respond to B. pertussis antigens in respiratory tissues when compared with aP-primed donors. Our findings demonstrate that wP vaccines induce CD4 TRM cells that can persist in respiratory tissues for decades.
目的是确定在儿童期接受全细胞百日咳（wP）或无细胞百日咳（aP）疫苗免疫的成年人的呼吸道组织中是否存在抗原特异性组织驻留记忆 T（TRM）细胞。从扁桃体或鼻组织细胞中分离出单核细胞，用百日咳博德特氏菌抗原培养，并通过流式细胞术定量 TRM 细胞。与 aP 疫苗接种的供体相比，儿童时期接受 wP 疫苗免疫的成年人在呼吸道组织中对百日咳博德特氏菌抗原产生白细胞介素 17A（IL-17A）和干扰素-γ（IFN-γ）的 TRM 细胞显著增加。我们的研究结果表明，wP 疫苗可诱导 CD4 TRM 细胞，这些细胞可在呼吸道组织中持续存在数十年。
