Wang Guoze, Du Kun, Xie Zhenjian, Tang Renyong, Jia Xianbo, Chen Shiyi, Lai Songjia
Farm Animal Genetic Resources Exploration and Innovation Key Laboratory of Sichuan Province, Sichuan Agricultural University, Chengdu 611130, People's Republic of China.
College of Food Science, Guizhou Medical University, Guiyang 550025, People's Republic of China.
Diabetes Metab Syndr Obes. 2020 Dec 1;13:4669-4680. doi: 10.2147/DMSO.S284246. eCollection 2020.
Rabbit is a good model for genetic and medical studies in other livestock species. The rabbit shows low adipose tissue deposition, and the phenomena indicates that there is some specificity of adipose deposition during the rabbit growth. However, little is known about genes that regulate the growth of adipose tissue in rabbits.
Deep RNA-seq and comprehensive bioinformatics analyses were used to characterize the genes of rabbit visceral adipose tissue (VAT) at 35, 85 and 120 days after birth. Differentially expressed genes (DEGs) were identified at the three growth stages by DESeq. To explore the function of the candidate genes, Gene Ontology (GO) enrichment and Kyoto Encyclopedia of Genes and Genomes (KEGG) pathway analyses were performed. Six DEGs were randomly selected, and their expression profiles were validated by q-PCR.
A total of 20,303 known transcripts and 99,199 new transcripts from 8 RNA sequencing libraries were identified, and 34 differentially expressed genes (DEGs) were screened. GO enrichment and KEGG pathway analyses revealed that the DEGs were mainly involved in lipid metabolism regulation including acylglycerol metabolic process and mobilization, and decomposition of lipids to generate ATP in adipocytes and fatty acid metabolism, included and . In addition, 133 protein-coding genes that play a role in adipose growth and development were screened, including acyl-CoA synthetase long-chain family member 5 () and fatty acid-binding protein 2 (). The validation results of six DEGs by q-PCR showed similar trends with the results of RNA-seq.
In summary, this study provides the first report of the coding genes profiles of rabbit adipose tissue during different growth stages. These data allow for the identification of candidate genes for subsequent studies on rabbit genetics and regulation of adipose cells, and provide an animal model for studying obesity in humans.
兔子是其他家畜物种遗传和医学研究的良好模型。兔子显示出低脂肪组织沉积，这一现象表明兔子生长过程中脂肪沉积存在一些特异性。然而，关于调节兔子脂肪组织生长的基因知之甚少。
采用深度RNA测序和综合生物信息学分析来表征出生后35、85和120天兔子内脏脂肪组织（VAT）的基因。通过DESeq在三个生长阶段鉴定差异表达基因（DEG）。为了探索候选基因的功能，进行了基因本体论（GO）富集和京都基因与基因组百科全书（KEGG）通路分析。随机选择6个DEG，通过q-PCR验证其表达谱。
从8个RNA测序文库中鉴定出总共20303个已知转录本和99199个新转录本，并筛选出34个差异表达基因（DEG）。GO富集和KEGG通路分析表明，DEG主要参与脂质代谢调节，包括酰基甘油代谢过程和动员，以及脂肪细胞中脂质分解以产生ATP和脂肪酸代谢，包括 和 。此外，筛选出133个在脂肪生长和发育中起作用的蛋白质编码基因，包括酰基辅酶A合成酶长链家族成员5（ ）和脂肪酸结合蛋白2（ ）。6个DEG的q-PCR验证结果与RNA测序结果显示出相似趋势。
总之，本研究首次报道了兔子不同生长阶段脂肪组织的编码基因谱。这些数据有助于鉴定后续兔子遗传学和脂肪细胞调节研究的候选基因，并为研究人类肥胖提供动物模型。
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