College of Agriculture and Animal Husbandry, Qinghai University, Xining, Qinghai Province 810016, People's Republic of China.
College of Animal Science and Technology, Northwest A&F University, Yangling, Shaanxi 712100, People's Republic of China; College of Food Science, South China Agricultural University, Guangzhou, 510642, China.
Gene. 2023 Jan 30;851:147031. doi: 10.1016/j.gene.2022.147031. Epub 2022 Nov 4.
The objective of this study was to evaluate the effect of the level of wheat substitution for corn on the fat metabolism of Tibetan lamb. A total of 90 Tibetan lambs [body weight (BW) of 19.78 ± 2.45 kg] were arranged with three substitution levels of wheat: WC (100 % corn), WL (10 % wheat substitution for corn), and WH (15 % wheat substitution for corn) on a dry matter basis. After the experiment, cry section technology was used to scrutinize the subcutaneous adipose tissue morphology, and genes related to fat metabolism were excavated using high-throughput sequencing technology. According to the study results, fat diameter and fat biovolume of the WL and WH groups were less than the WC group. A total of 506 differentially expressed genes (DEGs) were identified by RNA sequencing (RNA-Seq) technology. Compared with the WC group, 66 DEGs were upregulated and 59 DEGs were downregulated in the WL group, and 179 DEGs were upregulated and 269 DEGs were downregulated in the WH group. The top 20 DEGs were analyzed by Gene Ontology (GO) enrichment analysis and Kyoto Encyclopedia of Genes and Genomes (KEGG) metabolic pathway, indicating significant differences in the fat metabolism pathway. Five DEGs were randomly screened for quantitative reverse transcription-polymerase chain reaction (qRT-PCR) verification, and the results were consistent with the RNA-Seq results, which proved the accuracy of sequencing. In summary, with the increase in the proportion of supplemental wheat, the fat cells became smaller, and the genes related to fat decomposition were significantly upregulated.
本研究旨在评估小麦替代玉米水平对藏羊脂肪代谢的影响。共选用 90 只藏羊[体重（BW）为 19.78±2.45kg]，设计 3 个小麦替代水平：WC（100%玉米）、WL（10%小麦替代玉米）和 WH（15%小麦替代玉米）。实验结束后，采用冷冻切片技术观察皮下脂肪组织形态，利用高通量测序技术挖掘与脂肪代谢相关的基因。研究结果表明，WL 和 WH 组的脂肪直径和脂肪生物量均小于 WC 组。RNA 测序（RNA-Seq）技术共鉴定出 506 个差异表达基因（DEGs）。与 WC 组相比，WL 组有 66 个上调 DEGs 和 59 个下调 DEGs，WH 组有 179 个上调 DEGs和 269 个下调 DEGs。对前 20 个差异表达基因进行基因本体（GO）富集分析和京都基因与基因组百科全书（KEGG）代谢通路分析，表明脂肪代谢通路存在显著差异。随机筛选 5 个 DEGs 进行定量逆转录聚合酶链反应（qRT-PCR）验证，结果与 RNA-Seq 结果一致，证明了测序的准确性。综上所述，随着补充小麦比例的增加，脂肪细胞变小，与脂肪分解相关的基因显著上调。
