生姜散对高脂饮食诱导的非酒精性脂肪性肝病肠道菌群的影响。
Effect of Sheng-Jiang Powder on Gut Microbiota in High-Fat Diet-Induced NAFLD.
作者信息
Li Juan, Hu Qian, Xiao-Yu Dai, Zhu Lv, Miao Yi-Fan, Kang Hong-Xin, Zhao Xian-Lin, Yao Jia-Qi, Long Dan, Tang Wen-Fu, Wan Mei-Hua
机构信息
Department of Integrated Traditional Chinese and Western Medicine, West China Hospital, Sichuan University, Chengdu 610041, Sichuan Province, China.
Key Laboratory of Transplant Engineering and Immunology, Sichuan University, Chengdu 610041, Sichuan Province, China.
出版信息
Evid Based Complement Alternat Med. 2020 Nov 26;2020:6697638. doi: 10.1155/2020/6697638. eCollection 2020.
BACKGROUND AND AIMS
Nonalcoholic fatty liver disease (NAFLD) is an alarming global health problem that is predicted to be the major cause of cirrhosis, hepatocellular carcinoma, and liver transplantation by next decade. Gut microbiota have been revealed playing an important role in the pathogenesis of NAFLD. Sheng-Jiang Powder (SJP), an empirical Chinese medicine formula to treat NAFLD, showed great hepatoprotective properties, but the impact on gut microbiota has never been identified. Therefore, we performed this study to investigate the effect of SJP on gut microbiota in NAFLD mice.
METHODS
NAFLD was induced by 12 weeks' high-fat diet (HFD) feeding. Mice were treated with SJP/normal saline daily for 6 weeks. Blood samples were obtained for serum biochemical indices and inflammatory cytokines measurement. Liver tissues were obtained for pathological evaluation and oil red O staining. The expression of lipid metabolism-related genes was quantified by RT-PCR and Western blotting. Changes in gut microbiota composition were analyzed by the 16s rDNA sequencing technique.
RESULTS
HFD feeding induced significant increase in bodyweight and serum levels of TG, TC, ALT, and AST. The pathological examination revealed obvious hepatic steatosis in HFD feeding mice. Coadministration of SJP effectively protected against bodyweight increase and lipid accumulation in blood and liver. Increased expression of PPAR mRNA was observed in HFD feeding mice, but a steady elevation of PPAR protein level was only found in SJP-treated mice. Meanwhile, the expression of FASN was much higher in HFD feeding mice. Microbiome analysis revealed obvious changes in gut microbiota composition among diverse groups. SJP treatment modulated the relative abundance of short-chain fatty acids (SCFAs) producing bacteria, including norank-f- and .
CONCLUSIONS
SJP is efficient in attenuating HFD-induced NAFLD, and it might be partly attributed to the regulation of gut microbiota.
背景与目的
非酒精性脂肪性肝病（NAFLD）是一个令人担忧的全球健康问题，预计在下个十年将成为肝硬化、肝细胞癌和肝移植的主要原因。肠道微生物群已被证明在NAFLD的发病机制中起重要作用。升降散（SJP）是一种治疗NAFLD的经验性中药方剂，具有很强的肝脏保护作用，但对肠道微生物群的影响尚未明确。因此，我们进行了本研究，以探讨SJP对NAFLD小鼠肠道微生物群的影响。
方法
通过12周的高脂饮食（HFD）诱导NAFLD。小鼠每天用SJP/生理盐水治疗6周。采集血样用于检测血清生化指标和炎性细胞因子。获取肝组织进行病理评估和油红O染色。通过RT-PCR和蛋白质印迹法对脂质代谢相关基因的表达进行定量分析。采用16s rDNA测序技术分析肠道微生物群组成的变化。
结果
HFD喂养导致体重以及血清甘油三酯（TG）、总胆固醇（TC）、谷丙转氨酶（ALT）和谷草转氨酶（AST）水平显著升高。病理检查显示HFD喂养的小鼠有明显的肝脂肪变性。联合使用SJP可有效防止体重增加以及血液和肝脏中的脂质积累。在HFD喂养的小鼠中观察到过氧化物酶体增殖物激活受体（PPAR）mRNA表达增加，但仅在SJP治疗的小鼠中发现PPAR蛋白水平持续升高。同时，HFD喂养的小鼠中脂肪酸合酶（FASN）的表达要高得多。微生物组分析显示不同组之间肠道微生物群组成有明显变化。SJP治疗调节了包括norank-f-和 在内的短链脂肪酸（SCFAs）产生菌的相对丰度。
结论
SJP能有效减轻HFD诱导的NAFLD，这可能部分归因于对肠道微生物群的调节。
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