Department of Zoology and Environmental Management, Faculty of Science, University of Kelaniya, Sri Lanka.
Department of Biosystems Engineering, Faculty of Agriculture and Plantation Management, Wayamba University, Sri Lanka.
Biomed Res Int. 2020 Nov 24;2020:1053818. doi: 10.1155/2020/1053818. eCollection 2020.
is a major vector of arboviruses that may be controlled on an area-wide basis, using novel approaches such as Sterile Insect Technique (SIT) and Incompatible Insect Technique (IIT). Larval diet is a critical factor to be considered in mass rearing of mosquitoes for SIT and IIT programs. Therefore, the current study is aimed at evaluating the effects of two novel diets developed from dry fish powder on the growth and development of immature stages and adult fitness-related characteristics of in Sri Lanka.
Three batches of the first instar larva, each containing 250 larvae, were exposed to three different larval diets as standard dry fish powder (D), dry fish powder meal and brewer's yeast (D), and International Atomic Energy Agency- (IAEA-) recommended diet (D), separately. Morphometric and developmental parameters of the 4 instar larvae, pupae, and adult mosquitoes reared under different dietary treatments were measured. The entire experimental setup was replicated thrice. A General Linear Model (GLM) in the form of two-way ANOVA was used for the statistical analysis.
Significant diet-based variations were observed in the head length, head width, thoracic length, thoracic width, abdominal length, abdominal width, and total length ( > 4.811; < 0.05) of larvae. The highest pupation success and the larval size were observed from the larvae fed the D diet, while the lowest was reported from D. All adult morphometric parameters of adult male and female mosquitoes also denoted significant dietary variations, reporting the best-sized adults from the D diet ( > 3.54; < 0.05). Further, significantly higher fecundity and male longevity were also shown by the adult ( > 7.897; < 0.01) mosquitoes reared under diet D.
Based on all the growth and developmental parameters, the D diet tends to perform similar to the IAEA-recommended diet in mass rearing of mosquitoes, while being more inexpensive. Therefore, larval diet D could be suggested as the ideal diet for mass rearing of for IIT and SIT-based vector control in Sri Lanka.
是虫媒病毒的主要载体，可通过使用新方法，如不育昆虫技术（SIT）和不相容昆虫技术（IIT）进行区域范围的控制。幼虫的饮食是大规模饲养蚊子用于 SIT 和 IIT 项目的一个关键因素。因此，本研究旨在评估两种从干鱼粉开发的新型饲料对斯里兰卡 幼虫的生长发育和成虫与适应相关特征的影响。
三批第一龄 幼虫，每批含 250 条幼虫，分别暴露于三种不同的幼虫饮食，即标准干鱼粉（D）、干鱼粉和酵母（D）和国际原子能机构（IAEA）推荐的饮食（D）。在不同饮食处理下饲养的 4 龄幼虫、蛹和成年蚊子的形态和发育参数进行了测量。整个实验设置重复了三次。使用双因素方差分析（ANOVA）的一般线性模型（GLM）进行统计分析。
幼虫的头部长度、头部宽度、胸部长度、胸部宽度、腹部长度、腹部宽度和全长（ > 4.811； < 0.05）均观察到基于饮食的显著差异。从 D 饮食喂养的幼虫中观察到最高的化蛹成功率和幼虫大小，而从 D 饮食喂养的幼虫中观察到最低的化蛹成功率和幼虫大小。成年雄性和雌性 蚊子的所有成年形态参数也表示出显著的饮食变化，从 D 饮食中报告了最大的成年蚊子（ > 3.54； < 0.05）。此外，从 D 饮食中饲养的成年 蚊子（ > 7.897； < 0.01）还表现出显著更高的生育力和雄性寿命。
根据所有的生长和发育参数，D 饮食在大规模饲养 蚊子方面表现类似于 IAEA 推荐的饮食，同时价格更便宜。因此，D 幼虫饮食可被建议作为斯里兰卡大规模饲养 蚊子用于 IIT 和 SIT 为基础的媒介控制的理想饮食。
