Oh Jong-Nam, Son Dongchan, Choi Kwang-Hwan, Hwang Jae Yeon, Lee Dong-Kyung, Kim Seung-Hun, Lee Mingyun, Jeong Jinsol, Choe Gyung Cheol, Lee Chang-Kyu
Department of Agricultural Biotechnology, Animal Biotechnology Major, and Research Institute for Agriculture and Life Sciences, Seoul National University, Seoul 08826, Korea.
Department of Cellular and Molecular Physiology, Yale School of Medicine, New Haven, CT 06510, USA.
Data Brief. 2020 Nov 21;33:106563. doi: 10.1016/j.dib.2020.106563. eCollection 2020 Dec.
Typical models of pluripotency, humans and mice, have been used to analyse the characteristics of pluripotent stem cells. However, these species exhibit molecular differences in many aspects. With similar physiology and genomics as humans, pigs are promising model for the research of pluripotency. The data of porcine pluripotent cells would be helpful in understanding the molecular network of human pluripotency. Pluripotent cells of humans and mice exhibit specific MicroRNA (miRNA) expression patterns to maintain the pluripotent state. Information about miRNA expression in pig pluripotent cells is not sufficient, so we analysed miRNAs in pluripotent (blastocysts and ES-like) and somatic cell samples (PEB and PFF). We screened cell-type specific miRNAs and identified their target genes. Functional annotation of the target genes was also conducted. Our data may facilitate miRNA-based induction and maintenance of the pluripotent state of porcine cells and provide support to fill the gap between the pluripotency networks of humans and mice.
多能性的典型模型，如人类和小鼠，已被用于分析多能干细胞的特征。然而，这些物种在许多方面表现出分子差异。猪与人类具有相似的生理学和基因组学，是研究多能性的有前途的模型。猪多能细胞的数据将有助于理解人类多能性的分子网络。人类和小鼠的多能细胞表现出特定的微小RNA（miRNA）表达模式以维持多能状态。关于猪多能细胞中miRNA表达的信息不足，因此我们分析了多能（囊胚和类胚胎干细胞）和体细胞样本（猪子宫内膜上皮细胞和猪胎儿成纤维细胞）中的miRNA。我们筛选了细胞类型特异性miRNA并鉴定了它们的靶基因。还对靶基因进行了功能注释。我们的数据可能有助于基于miRNA诱导和维持猪细胞的多能状态，并为填补人类和小鼠多能性网络之间的空白提供支持。
