Norlund R, Roos N, Täljedal I B
Department of Histology and Cell Biology, University of Umeå, Sweden.
Biosci Rep. 1987 Nov;7(11):859-69. doi: 10.1007/BF01119477.
Quantitative X-ray microanalysis of 8 elements was performed on ultrathin, freeze-dried sections of islets and pancreas pieces from non-inbred ob/ob-mice. Diffusion of elements was reduced to a minimum by rapidly freezing the tissue samples between nitrogen-cooled polished copper surfaces and avoiding the use of chemical fixatives and stains. The ultrastructural morphology was adequately maintained to allow measurements on secretory granules, mitochondria, cell nuclei, and cytoplasm free of these organelles. The distribution of the various elements between cellular compartments was similar in islet beta-cells and exocrine pancreas cells. However, the insulin secretory granules were outstanding in exhibiting the highest concentrations of zinc and calcium. In comparison with cytoplasm in the beta-cells, the insulin granules accumulated calcium 2-fold and zinc as much as 40-fold. As no correlation could be made for endoplasmic reticulum in the cytoplasmic measurements areas, the true accumulations above cytosol are likely to be even higher.
对非近交系ob/ob小鼠的胰岛和胰腺组织超薄冷冻干燥切片进行了8种元素的定量X射线微分析。通过在液氮冷却的抛光铜表面之间快速冷冻组织样本，并避免使用化学固定剂和染色剂，将元素扩散降至最低。超微结构形态得到充分维持，以便对分泌颗粒、线粒体、细胞核和不含这些细胞器的细胞质进行测量。胰岛β细胞和外分泌胰腺细胞中各元素在细胞区室之间的分布相似。然而，胰岛素分泌颗粒中锌和钙的浓度最高。与β细胞的细胞质相比，胰岛素颗粒积累的钙是其2倍，锌是其40倍。由于在细胞质测量区域无法对内质网进行相关性分析，因此高于细胞质溶胶的真实积累量可能更高。
