Grupo de Investigación «Farmacognosia y Medicina Tradicional», Universidad Nacional Mayor de San Marcos, Lima, Perú.
Centro de Investigaciones Tecnológicas, Biomédicas y Medioambientales, Universidad Nacional Mayor de San Marcos, Lima, Perú.
Rev Peru Med Exp Salud Publica. 2020 Dec 2;37(3):454-461. doi: 10.17843/rpmesp.2020.373.4817.
To determine the in vitro cytotoxic and genotoxic effect of the crude and ethanolic extract from the Curcuma longa L. rhizome.
The cytotoxic effect was evaluated using DU-145, HT-29, 3T3 BALB/c cell lines. The growth percentages in 48 hours; and the half maximal inhibitory concentration (IC50) were determined. The genotoxic effect on human genomic DNA was determined using the Tomasevich method.
Crude extract produced an IC50 of 12.98 ± 0.21 μg/mL for the HT-29 tumor cell line, which is lower than the value obtained for DU-145, with an IC50 of 36.77 ± 9.12 μg/mL. The ethanolic extract presented an IC50 of 13.24 ± 0.77 and 20.54 ± 2.58 μg/mL for both cell lines, respectively; the curcumin standard compound presented an IC50 of 3.96 ± 0.60 and 13.94 ± 2.79 μg/mL, respectively. Crude extract concentrations of 50 and 100 mg/mL fragmented between 40% to 95% of human genomic DNA; while at 200 mg/mL, fragmentation was greater than 95%. The ethanolic extract at all concentrations did not fragment the DNA. Curcumin at 200 mg/mL fragmented less than 5% of human genomic DNA.
The crude and ethanolic extracts of Curcuma longa L. demonstrate different in vitro cytotoxic effects for the human tumor cell lines DU-145 and HT-29; similar to the standard curcumin compound. The crude extract of Curcuma longa L. shows a potent genotoxic in vitro activity against human genomic DNA; this type of effect is not produced by the ethanolic extract.
测定姜黄根茎的粗提物和乙醇提取物的体外细胞毒性和遗传毒性。
采用 DU-145、HT-29、3T3 BALB/c 细胞系评估细胞毒性作用。测定 48 小时的生长百分率和半最大抑制浓度（IC50）。采用 Tomasevich 法测定对人基因组 DNA 的遗传毒性作用。
粗提物对 HT-29 肿瘤细胞系的 IC50 为 12.98 ± 0.21μg/mL，低于 DU-145 的 IC50（36.77 ± 9.12μg/mL）。乙醇提取物对两种细胞系的 IC50 分别为 13.24 ± 0.77μg/mL 和 20.54 ± 2.58μg/mL；姜黄素标准化合物的 IC50 分别为 3.96 ± 0.60μg/mL 和 13.94 ± 2.79μg/mL。粗提物浓度为 50 和 100mg/mL 时，可使 40%至 95%的人基因组 DNA 片段化；而在 200mg/mL 时，片段化大于 95%。所有浓度的乙醇提取物均未使 DNA 片段化。姜黄素在 200mg/mL 时使少于 5%的人基因组 DNA 片段化。
姜黄的粗提物和乙醇提取物对人肿瘤细胞系 DU-145 和 HT-29 表现出不同的体外细胞毒性作用；与标准姜黄素化合物相似。姜黄的粗提取物对人基因组 DNA 具有较强的体外遗传毒性作用；这种作用不会由乙醇提取物产生。
