DÜz MÜrÜvvet, DoĞan YaĞmur Nİl, DoĞan İlkay
Department of Chemistry, Faculty of Arts and Sciences, Afyon Kocatepe University, 03200, Afyonkarahisar, Turkey.
Gaziantep University Islahiye Vocational School, 27800, Gaziantep, Turkey.
An Acad Bras Cienc. 2020 Dec 7;92(4):e20200105. doi: 10.1590/0001-3765202020200105. eCollection 2020.
In recent years, it is very important to find more safe and natural antioxidants than biological sources to replace synthetic antioxidants. The antioxidant properties of 22 lactic acid bacteria were investigated in vitro in the present study. The conducted in vitro antioxidant assays included scavenging the 2,2-diphenyl-1-picrylhydrazil (DPPH) free radical, metal (Fe+2) ion chelation, hydroxyl radical and superoxide radical scavenging properties, and anti-lipid peroxidation. Analysis of DPPH free radical scavenging property of microorganisms demonstrated that Lactobacillus plantarum IH14L (90.34 ± 0.40%) strain exhibited the highest activity. The highest Fe+2 chelation activity was observed with Lactobacillus curvatus GH5L (75.98 ± 5.35%), while the lowest chelation activity was observed with L. plantarum IH14L (19.80 ± 0,05). The highest and lowest hydroxyl radical scavenging strains were L. plantarum IH16L (82.25 ± 1.60%) and L. plantarum IH26L (35.60 ± 4.50) strains, respectively. Comparison of superoxide radical scavenging activities of the microorganisms demonstrated that these activities ranged between 21.63 ± 1.32% and 7.22 ± 0.04%. A positive correlation was identified between the hydroxyl radical scavenging activity of the microorganisms and the anti-lipid peroxidation property. It was found that they had the potential for use in nutritional and probiotic applications as protective and natural antioxidants.
近年来，寻找比生物源更安全、天然的抗氧化剂来替代合成抗氧化剂非常重要。本研究在体外对22株乳酸菌的抗氧化性能进行了研究。所进行的体外抗氧化试验包括清除2,2-二苯基-1-苦基肼（DPPH）自由基、金属（Fe²⁺）离子螯合、羟基自由基和超氧阴离子自由基清除性能以及抗脂质过氧化作用。对微生物DPPH自由基清除性能的分析表明，植物乳杆菌IH14L菌株（90.34±0.40%）表现出最高活性。弯曲乳杆菌GH5L表现出最高的Fe²⁺螯合活性（75.98±5.35%），而植物乳杆菌IH14L的螯合活性最低（19.80±0.05）。羟基自由基清除能力最高和最低的菌株分别是植物乳杆菌IH16L（82.25±1.60%）和植物乳杆菌IH26L（35.60±4.50）。对微生物超氧阴离子自由基清除活性的比较表明，这些活性在21.63±1.32%至7.22±0.04%之间。微生物的羟基自由基清除活性与抗脂质过氧化性能之间存在正相关。结果发现，它们有潜力作为保护性天然抗氧化剂用于营养和益生菌应用。
