Kuang Zheyi, Yan Xiaofang, Yuan Yanfei, Wang Ruiqi, Zhu Haifan, Wang Youyang, Li Jianfeng, Ye Jianwen, Yue Haitao, Yang Xiaofeng
School of Intelligence Science and Technology, Xinjiang University, Urumqi, 830017, China.
School of Biology and Biological Engineering, South China University of Technology, Guangzhou, 510006, China.
Synth Syst Biotechnol. 2024 Jun 28;9(4):793-808. doi: 10.1016/j.synbio.2024.06.008. eCollection 2024 Dec.
Microorganisms, particularly extremophiles, have evolved multiple adaptation mechanisms to address diverse stress conditions during survival in unique environments. Their responses to environmental coercion decide not only survival in severe conditions but are also an essential factor determining bioproduction performance. The design of robust cell factories should take the balance of their growing and bioproduction into account. Thus, mining and redesigning stress-tolerance elements to optimize the performance of cell factories under various extreme conditions is necessary. Here, we reviewed several stress-tolerance elements, including acid-tolerant elements, saline-alkali-resistant elements, thermotolerant elements, antioxidant elements, and so on, providing potential materials for the construction of cell factories and the development of synthetic biology. Strategies for mining and redesigning stress-tolerance elements were also discussed. Moreover, several applications of stress-tolerance elements were provided, and perspectives and discussions for potential strategies for screening stress-tolerance elements were made.
微生物，尤其是极端微生物，已经进化出多种适应机制，以应对在独特环境中生存期间遇到的各种应激条件。它们对环境胁迫的反应不仅决定了在恶劣条件下的生存能力，也是决定生物生产性能的一个重要因素。设计强大的细胞工厂应考虑到它们生长和生物生产之间的平衡。因此，挖掘和重新设计耐胁迫元件以优化细胞工厂在各种极端条件下的性能是必要的。在此，我们综述了几种耐胁迫元件，包括耐酸元件、耐盐碱元件、耐热元件、抗氧化元件等，为细胞工厂的构建和合成生物学的发展提供了潜在材料。还讨论了挖掘和重新设计耐胁迫元件的策略。此外，还介绍了耐胁迫元件的一些应用，并对筛选耐胁迫元件的潜在策略进行了展望和讨论。
