Lee Ga Hun, Choi Sung Hyun, Lee Yong Hyun, Park Jae Kweon
Department of Life Sciences, College of Bionano, Gachon University, Seongnamdaero 1342, Seongnam-si 461-701, Republic of Korea.
Nutrients. 2025 Jul 28;17(15):2464. doi: 10.3390/nu17152464.
BACKGROUND/OBJECTIVES: Green tripe (GRET) is rich in essential fatty acids, vitamins, calcium, phosphorus, and other nutrients and contains various beneficial microorganisms, including lactobacillus, along with feed components consumed by ruminants.
In this study, and were isolated from GRET and evaluated for their potential as probiotics, focusing on their anti-inflammatory properties and ability to modulate inflammatory responses.
When heat-killed or (10 CFU/mL) and their metabolites (0.5 mg/mL) were applied to RAW 264.7 macrophages stimulated with LPS, nitric oxide (NO) production was reduced by approximately 10-35% and 2-11%, respectively. Furthermore, the expression levels of key anti-inflammatory cytokines, TNF-α and IL-6, were suppressed by more than 5%. These effects were not due to cytotoxicity but instead due to genuine anti-inflammatory activity. In addition, both strains exhibited antioxidant activity, as demonstrated by their performance in ABTS and FRAP assays.
These findings suggest that and have significant antioxidant and anti-inflammatory properties, highlighting their potential as probiotics and prebiotics. Moreover, these newly isolated strains from GRET are expected to serve as valuable functional ingredients for developing health-promoting foods and dietary supplements.
背景/目的：生牛肚富含必需脂肪酸、维生素、钙、磷及其他营养物质，含有包括乳酸菌在内的多种有益微生物，以及反刍动物食用的饲料成分。
在本研究中，从生牛肚中分离出[具体菌株1]和[具体菌株2]，并评估它们作为益生菌的潜力，重点关注其抗炎特性和调节炎症反应的能力。
当将热灭活的[具体菌株1]或[具体菌株2]（10⁶CFU/mL）及其代谢产物（0.5 mg/mL）应用于用脂多糖刺激的RAW 264.7巨噬细胞时，一氧化氮（NO）的产生分别减少了约10 - 35%和2 - 11%。此外，关键抗炎细胞因子TNF-α和IL-6的表达水平被抑制了5%以上。这些作用并非由于细胞毒性，而是由于真正的抗炎活性。此外，两株菌均表现出抗氧化活性，这在ABTS和FRAP测定中得到了证明。
这些发现表明[具体菌株1]和[具体菌株2]具有显著的抗氧化和抗炎特性，突出了它们作为益生菌和益生元的潜力。此外，这些从生牛肚中新分离的菌株有望成为开发促进健康食品和膳食补充剂的有价值的功能成分。
