Key Laboratory of Artificial Micro- and Nano-Structures of Ministry of Education, School of Physics and Technology, Wuhan University, Wuhan 430072, China.
Department of Clinical Laboratory, Zhongnan Hospital of Wuhan University, Wuhan 430071, China.
Anal Chem. 2021 Jan 19;93(2):1033-1042. doi: 10.1021/acs.analchem.0c03933. Epub 2020 Dec 9.
Fetal nucleated red blood cells (fNRBCs) in maternal peripheral blood containing the whole genetic information of the fetus may serve for noninvasive pregnant diagnostics (NIPD). However, the fetal cell-based NIPD is seriously limited by the poor purity of the isolated fNRBCs. Recently, the biomimetic cell membrane-camouflaged nanoparticles containing outstanding features have been widely used to detect and isolate rare cells from the peripheral blood samples. In this work, enythrocyte (RBC) and leukocyte (WBC) membranes are fused and coated onto magnet nanoparticles and then modified with anti-CD147 to isolate fNRBCs from the maternal peripheral blood with significant efficiency (∼90%) and purity (∼87%) in simulated spiked blood samples. Further, fNRBCs were isolated and identified from a series of maternal peripheral blood samples coming from pregnant women of 11-13 gestational weeks, and different chromosomal aneuploidies were diagnosed using fNRBCs isolated from maternal blood in early pregnancy. Our strategy may offer additional opportunity to overcome the limitations of current cell-based NIPD platforms.
胎儿有核红细胞（fNRBCs）存在于母体外周血中，含有胎儿的全部遗传信息，可用于非侵入性产前诊断（NIPD）。然而，基于胎儿细胞的 NIPD 受到所分离的 fNRBCs 纯度较差的严重限制。最近，仿生细胞膜伪装纳米颗粒具有突出的特性，已被广泛用于从外周血样本中检测和分离稀有细胞。在这项工作中，红细胞（RBC）和白细胞（WBC）的细胞膜融合并包裹在磁性纳米颗粒上，然后用抗 CD147 进行修饰，以从模拟污染血样中以约 90%的显著效率和约 87%的纯度分离 fNRBCs。此外，还从来自妊娠 11-13 周孕妇的一系列外周血样本中分离和鉴定 fNRBCs，并使用早期妊娠母体外周血分离的 fNRBCs 诊断不同的染色体非整倍体。我们的策略可能为克服当前基于细胞的 NIPD 平台的局限性提供了额外的机会。
