Ou Pinghua, Hao Cong, Liu Jue, Yang Hailin, He Rengui, Zhang Taomei, Wang Yali, Ruan Jianming
State Key Laboratory of Powder Metallurgy, Central South University, Changsha 410083, People's Republic of China.
Department of Stomatology, The Third Xiangya Hospital, Central South University, Changsha 410013, People's Republic of China.
Biomed Mater. 2021 Mar 3;16(3). doi: 10.1088/1748-605X/abd1f8.
The aim of this study was to evaluate the biocompatibility and osteogenic potential of 50%Nb-Ti-Zr (NTZ,= 20%, 30%, 40% by weight) alloys as compared with dental commercial pure titanium (cpTi). Cell cytotoxicity assay, fluorescence microscopy and electron microscopy were used to measure thebiocompatibility of NTZ. The expression of alkaline phosphatase (ALP), integrin β1, osteocalcin (OC), Ki67 and collagen-I (Col-I) at the mRNA level was measured by real-time reverse transcription-polymerase chain reaction. Osseointegration ability was determined using x-ray evaluation and histological analysis. Compared with the MG63 cells grown on cpTi on day 3, the viability, adherence and proliferation rates of cells cultured on NTZ alloys were significantly improved (< 0.05). Furthermore, similar expression levels of Ki67, Col-I, OC and ALP were found in the MG63 cells grown on NTZ alloys and those grown on cpTi. The Cbfα1 level was significantly higher for the 50%Nb-30%Ti-Zr (NTZ3) than for the cpTi group on day 6 (< 0.01), indicating that NTZ alloys can induce osteogenesis. A considerable amount of new bone formation and osseointegration was observed around NTZ3 implants compared with cpTi implants. Collectively, NTZ3 showed superior biocompatibility and osteogenic activity; therefore, NTZ3 may be an excellent replacement for dental Ti implants.
本研究旨在评估50%铌-钛-锆（NTZ，重量百分比分别为20%、30%、40%）合金与牙科商用纯钛（cpTi）相比的生物相容性和成骨潜力。采用细胞毒性试验、荧光显微镜和电子显微镜来测量NTZ的生物相容性。通过实时逆转录-聚合酶链反应测量碱性磷酸酶（ALP）、整合素β1、骨钙素（OC）、Ki67和I型胶原（Col-I）在mRNA水平的表达。使用X射线评估和组织学分析来确定骨整合能力。与第3天在cpTi上生长的MG63细胞相比，在NTZ合金上培养的细胞的活力、黏附率和增殖率显著提高（<0.05）。此外，在NTZ合金上生长的MG63细胞和在cpTi上生长的细胞中发现了相似的Ki67、Col-I、OC和ALP表达水平。在第6天，50%铌-30%钛-锆（NTZ3）组的Cbfα1水平显著高于cpTi组（<0.01），表明NTZ合金可诱导成骨。与cpTi植入物相比，在NTZ3植入物周围观察到大量新骨形成和骨整合。总体而言，NTZ3表现出优异的生物相容性和成骨活性；因此，NTZ3可能是牙科钛植入物的极佳替代品。
