Chen Yi-Ming, Chen Po-Ku, Chang Ching-Kun, Lin Chi-Chen, Chen Hsin-Hua, Lan Joung-Liang, Chang Shih-Hsin, Chen Der-Yuan
Division of Allergy, Immunology and Rheumatology, Department of Medical Research, Taichung Veterans General Hospital, Taichung 40705, Taiwan.
Ph.D. Program in Translational Medicine & Rong Hsing Research Center for Translational Medicine, National Chung Hsing University, Taichung 40227, Taiwan.
Life (Basel). 2020 Dec 7;10(12):330. doi: 10.3390/life10120330.
Apolipoprotein E (ApoE) polymorphism and adipokines are linked to atherosclerosis. We aimed to investigate the associations of apoE genotypes with adipokines, inflammatory parameters, and cardiovascular disease (CVD) risks in rheumatoid arthritis (RA) patients. We enrolled 152 RA patients and 49 healthy control (HC) subjects. The apoE genotyping was determined by a polymerase chain reaction, while plasma levels of adipokines and inflammatory cytokines were measured with ELISA. Although apoE genotypes distributions were indistinguishable between RA patients and HC, we found significantly higher levels of apoE and adipokines in RA patients compared with HC. RA patients with ε2ε3 genotype had lower levels of TNF-α, IL-6, resistin, and visfatin, but higher leptin levels compared with ε3ε3 genotype patients. Patients with ε3ε4 genotype had significantly higher low-density lipoprotein-cholesterol (LDL-C) levels and atherogenic index scores compared with ε2ε3 genotype carriers. Moreover, patients with ε2ε3 genotype had significantly lower 10-year CVD risk than ε3ε3 or ε3ε4 genotype patients. ε3ε4 genotype and adiponectin levels were independent predictors of a high 10-year CVD risk. RA patients with ε2ε3 genotype are associated with lower levels of TNF-α, IL-6, resistin, visfatin, and CVD risk, while RA patients with ε3ε4 genotype exhibited higher levels of LDL-C, insulin resistance, and higher CVD risks.
载脂蛋白E（ApoE）多态性与脂肪因子与动脉粥样硬化有关。我们旨在研究类风湿关节炎（RA）患者中ApoE基因型与脂肪因子、炎症参数和心血管疾病（CVD）风险之间的关联。我们纳入了152例RA患者和49例健康对照（HC）受试者。通过聚合酶链反应确定ApoE基因分型，同时用酶联免疫吸附测定法测量血浆中脂肪因子和炎症细胞因子的水平。虽然RA患者和HC之间的ApoE基因型分布没有差异，但我们发现RA患者的ApoE和脂肪因子水平明显高于HC。与ε3ε3基因型患者相比，ε2ε3基因型的RA患者TNF-α、IL-6、抵抗素和内脂素水平较低，但瘦素水平较高。与ε2ε3基因型携带者相比，ε3ε4基因型患者的低密度脂蛋白胆固醇（LDL-C）水平和动脉粥样硬化指数得分显著更高。此外，ε2ε3基因型患者的10年CVD风险明显低于ε3ε3或ε3ε4基因型患者。ε3ε4基因型和脂联素水平是10年高CVD风险的独立预测因素。ε2ε3基因型的RA患者与较低的TNF-α、IL-6、抵抗素、内脂素水平和CVD风险相关，而ε3ε4基因型的RA患者表现出较高的LDL-C水平、胰岛素抵抗和较高的CVD风险。
