Zou L, Li Y, Zhou G L, Zhen B J, Zhang P, Jiang N, Zhao F L, Wang J G, Li H J, Wang Y C, Zhang G F, Zhang M J
Beijing Tongzhou District Center for Disease Control and Prevention, Beijing 101100, China.
Beijing Shunyi District Center for Disease Control and Prevention, Beijing 101300, China; Workstation for Microbial Infectious Disease, Beijing Shunyi District Center for Disease Control and Prevention, Beijing 101300, China.
Zhonghua Liu Xing Bing Xue Za Zhi. 2020 Oct 10;41(10):1692-1696. doi: 10.3760/cma.j.cn112338-20200109-00025.
To understand the etiological characteristics of an acute gastroenteritis outbreak. Real-time polymerase chain reaction (PCR) and bacteria cultures were performed for the samples, including stool samples from patients and cooks, environmental swabs, raw food material (chicken meat), collected during the outbreak. Pulsed-field gel electrophoresis, antibiotics susceptibility test and whole-genome sequencing were performed for the isolates. Four stool samples from patients were positive for by real-time PCR, in which 1 strain was isolated from a case who had no antibiotic treatment. Twelve and 7 isolates were obtained from 4 raw chicken meat samples. The strain isolated from the case was resistant to nalidixic acid, ciprofloxacin, chloramphenicol, florfenicol and tetracycline. The MLST analysis with the whole-genome sequences confirmed that the isolate from the case belonged to ST10075. Antimicrobial resistance genes and were found in the genome of the isolate from the patient by the whole-genome sequencing. No mutation in 23S rRNA was found and the C257T mutation in was identified in this isolate. Laboratory analysis indicated that infection might be the major cause of this gastroenteritis outbreak.
为了解一起急性胃肠炎暴发的病因特征。对暴发期间采集的样本进行了实时聚合酶链反应（PCR）和细菌培养，样本包括患者和厨师的粪便样本、环境拭子、生食品原料（鸡肉）。对分离株进行了脉冲场凝胶电泳、抗生素敏感性试验和全基因组测序。4例患者的粪便样本经实时PCR检测呈阳性，其中1株分离自未接受抗生素治疗的病例。从4份生鸡肉样本中获得了12株和7株分离株。从该病例分离出的菌株对萘啶酸、环丙沙星、氯霉素、氟苯尼考和四环素耐药。对全基因组序列进行的多位点序列分型分析证实，从该病例分离出的菌株属于ST10075。通过全基因组测序在患者分离株的基因组中发现了抗菌耐药基因和。未发现23S rRNA突变，该分离株中发现了的C257T突变。实验室分析表明，感染可能是此次胃肠炎暴发的主要原因。
