Department of Biotechnology, Microbiology and Human Nutrition, University of Life Sciences in Lublin, 20-704 Lublin, Poland.
Institute of Plant Genetics, Breeding and Biotechnology, University of Life Sciences in Lublin, 20-950 Lublin, Poland.
Plant Dis. 2021 Feb;105(2):251-254. doi: 10.1094/PDIS-02-20-0318-SC. Epub 2020 Dec 9.
is a fungal pathogen causing losses in wheat yields. Here, we present new primer sets for species-specific identification of this microorganism in wheat leaf samples using conventional PCR. Primer sets were validated in silico using tools available in genetic databases. Furthermore, in vitro tests were also carried out on 190 common wheat samples with visual symptoms of Septoria tritici blotch (STB) collected in Poland in three growing seasons (2015, 2016, 2017). The designed primer sets showed full hybridization to the available genetic resources deposited in the NCBI GenBank database, and their high specificity and sensitivity were demonstrated on wheat leaf samples and selected fungal strains.
是一种引起小麦减产的真菌病原体。在这里，我们使用常规 PCR 提供了新的引物对，用于在小麦叶片样本中对这种微生物进行种特异性鉴定。使用遗传数据库中提供的工具对引物对进行了计算机模拟验证。此外，还在三个生长季节（2015、2016 和 2017 年）在波兰收集的有小麦叶枯病（STB）症状的 190 个普通小麦样本上进行了体外测试。设计的引物对与保存在 NCBI GenBank 数据库中的可用遗传资源完全杂交，并且在小麦叶片样本和选定的真菌菌株上证明了其高度特异性和敏感性。
