Association of II Polymorphism in and (894G/T and -786T/C) Polymorphisms in eNOS Gene With Stroke Susceptibility in Tunisian Patients α2 Gene Polymorphism in α2β1 Integrin and eNOS Gene Variants and Stroke.

BACKGROUND

This study investigated the association of II polymorphism in α2β1 integrin gene () and eNOS (894G/T and -786T/C) polymorphisms with ischemic stroke (IS) in Tunisian patients.

METHODS

The study comprised 210 patients with IS and 208 controls. The genotypes of the II polymorphism in and eNOS (894G/T and -786T/C) polymorphisms were determined using the PCR-RFLP. The χ test was used and the genotype data comparison included heterozygous groups. Haplotype estimation and multiple logistic regression analysis were performed to analyze the significance of polymorphisms.

RESULTS

The genotype distribution of the II polymorphism was significantly different between cases and controls ( 0.004). This polymorphism was associated with the risk of IS ( = 3.38, 0.001) for the II(+/+) genotype. Likewise, the genotype distributions of (894G/T and -786T/C) polymorphisms were significantly different between the two groups ( 0.005 and 0.01, respectively). The 894G/T polymorphism increased the risk of IS for the TT genotype ( = 2.23, 0.008) and the GT genotype ( = 1.74, 0.009). In addition, the -786T/C variant in the gene was a risk factor for IS for CC homozygous ( = 2.52, 0.005). T-C Haplotype ( = 3.06) from combination of the eNOS (894G/T and -786T/C) and T-C-II(+) haplotype ( = 2.76) from combination of eNOS and polymorphisms represented high risks for IS.

CONCLUSIONS

This study suggests that the II variant in is associated with IS susceptibility. Furthermore, the 894G/T and -786T/C polymorphisms in the gene may be considered as genetic risk factors for IS in the Tunisian population.