Department of Cardiology, Jiangsu Taizhou People's Hospital, Taizhou 225300, China.
Dis Markers. 2020 Nov 22;2020:8872142. doi: 10.1155/2020/8872142. eCollection 2020.
To investigate the expression profiles of long noncoding RNAs (lncRNAs) in patients with atrial fibrillation (AF).
The peripheral blood monocytes of a total of 20 patients with AF and 20 healthy subjects were collected for gene chip technology to detect differentially expressed lncRNAs from 2017.01 to 2017.08. Reverse transcription polymerase chain reaction (RT-PCR) was applied for further verification. Gene Ontology (GO) and Kyoto Encyclopedia of Genes and Genomes (KEGG) analyses were performed to identify the functions of differentially expressed genes and related pathways.
There were 19 lncRNAs differentially expressed (FC ≥ 2, < 0.05), of which 6 were upregulated and 13 were downregulated. Two of three upregulated lncRNAs ( = 0.014 and 0.006 for HNRNPU-AS1 and LINC00861, respectively) and two of three downregulated lncRNAs ( = 0.028 and 0.032 for RP11-443B7.3 and CTD-2616J11.14, respectively) were randomly confirmed by RT-PCR and showed a significantly different expression with the RNA-seq results. GO analysis showed that differentially expressed genes enriched in differentially expressed transcripts in biological process were mainly involved in metabolic process, catabolic process, and biosynthetic process. Differentially expressed transcripts in cellular component were mainly involved in nuclear lumen, organelle lumen, and cytoplasm. Differentially expressed transcripts in molecular function were mainly involved in protein binding, RNA binding, and molecular function. KEGG enrichment pathway analysis showed that some of the enrichment pathways associated with differentially expressed lncRNAs include calcium signaling pathway, NF-kappa B signaling pathway, cytokine-cytokine receptor interaction, and Toll-like receptor signaling pathway. HNRNPU-AS1 was the highest positive correlated lncRNA in the networks.
The expression of lncRNA in peripheral blood of AF patients is different from that of normal people. The physiological functions of these differentially expressed lncRNAs may be related to the pathogenesis of AF, which provide experimental basis and new therapeutic target for prognosis and treatment of patients with AF. HNRNPU-AS1 may play an important role in the pathophysiology and mechanisms of AF.
探讨心房颤动（房颤）患者长链非编码 RNA（lncRNA）的表达谱。
2017 年 1 月至 2017 年 8 月，收集 20 例房颤患者和 20 例健康对照者的外周血单核细胞，采用基因芯片技术检测差异表达的 lncRNA。应用逆转录聚合酶链反应（RT-PCR）进一步验证。进行基因本体（GO）和京都基因与基因组百科全书（KEGG）分析，以鉴定差异表达基因及其相关通路的功能。
有 19 个 lncRNA 差异表达（FC≥2， < 0.05），其中 6 个上调，13 个下调。三个上调的 lncRNA 中有两个（HNRNPU-AS1 和 LINC00861 的 = 0.014 和 0.006），三个下调的 lncRNA 中有两个（RP11-443B7.3 和 CTD-2616J11.14 的 = 0.028 和 0.032）通过 RT-PCR 随机验证，其表达与 RNA-seq 结果有显著差异。GO 分析显示，差异表达基因在生物学过程中富集的差异表达转录本主要涉及代谢过程、分解代谢过程和生物合成过程。细胞成分中的差异表达转录本主要涉及核腔、细胞器腔和细胞质。分子功能中的差异表达转录本主要涉及蛋白结合、RNA 结合和分子功能。KEGG 富集通路分析显示，与差异表达 lncRNA 相关的一些富集通路包括钙信号通路、NF-κB 信号通路、细胞因子-细胞因子受体相互作用和 Toll 样受体信号通路。HNRNPU-AS1 是网络中相关性最高的正相关 lncRNA。
房颤患者外周血 lncRNA 的表达与正常人不同。这些差异表达的 lncRNA 的生理功能可能与房颤的发病机制有关，为房颤患者的预后和治疗提供了实验依据和新的治疗靶点。HNRNPU-AS1 可能在房颤的病理生理学和机制中发挥重要作用。
