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液相色谱-质谱联用分析生物分子中残留问题的故障排除：以神经肽Y为例

Troubleshooting Carry-Over in the LC-MS Analysis of Biomolecules: The Case of Neuropeptide Y.

作者信息

Yamagaki Tohru, Yamazaki Takashi

机构信息

Suntory Institute for Bioorganic Research, Suntory Foundation for Life Sciences, 8-1-1 Seikadai, Seika-cho, Soraku-gun, Kyoto 619-0284, Japan.

出版信息

Mass Spectrom (Tokyo). 2019;8(2):S0083. doi: 10.5702/massspectrometry.S0083. Epub 2020 Feb 26.

DOI:10.5702/massspectrometry.S0083
PMID:33299733
原文链接:https://pmc.ncbi.nlm.nih.gov/articles/PMC7709882/
Abstract

We describe systematic troubleshooting of the carry-over of neuropeptide Y (NPY) in LC-MS analysis. The objective was to remove candidate parts of the LC-MS system that are responsible for carry over one-by-one. The findings indicate that the carry-over of NPY occurs on the column, particularly in the guard column and at the consumable seals of the sample-needle and high-pressure valves. The methodology demonstrates that it is possible to troubleshoot carry-over in an LC-MS system in a systematic and logical manner.

摘要

我们描述了在液相色谱-质谱联用（LC-MS）分析中对神经肽Y（NPY）残留进行系统故障排查的过程。目的是逐一排查LC-MS系统中导致残留的可能部件。结果表明，NPY的残留发生在色谱柱上，尤其是在保护柱以及样品针和高压阀的易耗密封处。该方法表明，以系统且合乎逻辑的方式对LC-MS系统中的残留进行故障排查是可行的。

https://cdn.ncbi.nlm.nih.gov/pmc/blobs/26c8/7709882/bc7b57130cc8/massspectrometry-8-2-S0083-figure03.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/26c8/7709882/62763e527f48/massspectrometry-8-2-S0083-figure01.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/26c8/7709882/48497ef01dc6/massspectrometry-8-2-S0083-figure02.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/26c8/7709882/bc7b57130cc8/massspectrometry-8-2-S0083-figure03.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/26c8/7709882/62763e527f48/massspectrometry-8-2-S0083-figure01.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/26c8/7709882/48497ef01dc6/massspectrometry-8-2-S0083-figure02.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/26c8/7709882/bc7b57130cc8/massspectrometry-8-2-S0083-figure03.jpg

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