神经肽Y：脑肽的完整氨基酸序列。
Neuropeptide Y: complete amino acid sequence of the brain peptide.
作者信息
Tatemoto K
出版信息
Proc Natl Acad Sci U S A. 1982 Sep;79(18):5485-9. doi: 10.1073/pnas.79.18.5485.
Abstract
The amino acid sequence of neuropeptide Y, a 36-residue peptide recently isolated from porcine brain, has been determined by using high performance liquid chromatography for separation of its tryptic and chymotryptic fragments and subsequent sequence analysis of the isolated fragments by an improved dansyl Edman subtractive technique. The amino acid sequence of neuropeptide Y has been found to be: Tyr-Pro-Ser-Lys-Pro-Asp-Asn-Pro-Gly-Glu-Asp-Ala-Pro-Ala-Glu-Asp-Leu-Ala-Arg-Tyr -Tyr-Ser-Ala-Leu-Arg-His-Tyr-Ile-Asn-Leu-Ile-Thr-Arg-Gln-Arg-Tyr-NH2. Neuropeptide Y has a high degree of sequence homology with peptide YY (70%), the newly isolated porcine intestinal peptide, and pancreatic polypeptide (50%). It is therefore proposed that neuropeptide Y, peptide YY, and pancreatic polypeptide are members of a newly recognized peptide family.
摘要
神经肽Y是一种最近从猪脑中分离出的含36个氨基酸残基的肽，其氨基酸序列的确定方法如下：先用高效液相色谱法分离其胰蛋白酶和糜蛋白酶片段，然后通过改进的丹磺酰乙二胺消减技术对分离出的片段进行序列分析。已发现神经肽Y的氨基酸序列为：酪氨酰-脯氨酰-丝氨酰-赖氨酰-脯氨酰-天冬氨酰-天冬酰胺-脯氨酰-甘氨酰-谷氨酰-天冬氨酰-丙氨酰-脯氨酰-丙氨酰-谷氨酰-天冬氨酰-亮氨酰-丙氨酰-精氨酰-酪氨酰-酪氨酰-丝氨酰-丙氨酰-亮氨酰-精氨酰-组氨酰-酪氨酰-异亮氨酰-天冬酰胺-亮氨酰-异亮氨酰-苏氨酰-精氨酰-谷氨酰胺-精氨酰-酪氨酰-NH2。神经肽Y与新分离出的猪肠肽YY（70%）和胰多肽（50%）有高度的序列同源性。因此，有人提出神经肽Y、肽YY和胰多肽是一个新确认的肽家族的成员。
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