Xu Linfen, Huang Lingna, Lian Chengying, Xue Huili, Lu Yanfang, Chen Xiujuan, Xia Yong
Department of Gynaecology, Fujian Provincial Maternity and Children's Hospital, Fujian Medical University, Fuzhou, Fujian, China.
Department of Key Laboratory for Prenatal Diagnosis and Birth Defect, Fujian Provincial Maternity and Children's Hospital, Fujian Medical University, Fuzhou, Fujian, China.
Int J Genomics. 2020 Nov 20;2020:1764959. doi: 10.1155/2020/1764959. eCollection 2020.
Embryonic miscarriage severely affects the life quality and physical and mental state of pregnant women. However, the detailed mechanism underlying embryonic miscarriage is not fully understood. This study is aimed at analyzing embryonic miscarriage. We collected samples from 25 normal pregnant women and 25 embryonic miscarriage patients of similar age to analyze microbiota isolated from the vagina. Crude examination of the vagina isolates showed that compared with the control group, 80% of the embryonic miscarriage group contained a significantly lower number of , the major healthy microbe in the vagina. Furthermore, the levels of Th1 and Th2 secreted cytokine interleukin 2 (IL-2) and interleukin10 (IL-10), respectively, were examined. Results showed that the IL2 level was higher, and IL10 level was lower in the embryonic miscarriage group than in the control group, whereas the IL2/IL10 level was higher in the embryonic miscarriage group than in the control group. This finding suggested that the immune response was suppressed in the embryonic miscarriage group. To further dissect the microbiota of the vagina in the two groups, 16S rDNA sequencing was performed. Bioinformatics analysis showed that 1096 and 998 overlapped operational taxonomic units were identified from the embryonic miscarriage and control groups, respectively. At the genus level, the relative abundance of Fam_Finegoldia, Lac_Coprococcus_3, and Lac_Roseburia significantly differed in the embryonic miscarriage group. Overall, our analyses provided potential biomarkers for embryonic miscarriage and elucidated the causative relationship between microbiota and immune responses and may enable the possible diagnosis and therapeutics of early pregnancy loss.
胚胎流产严重影响孕妇的生活质量以及身心健康。然而，胚胎流产背后的详细机制尚未完全明确。本研究旨在分析胚胎流产情况。我们收集了25名正常孕妇和25名年龄相仿的胚胎流产患者的样本，以分析从阴道分离出的微生物群。对阴道分离物的初步检查显示，与对照组相比，胚胎流产组中有80%的乳酸杆菌数量显著减少，乳酸杆菌是阴道内主要的有益微生物。此外，还检测了分别由Th1和Th2分泌的细胞因子白细胞介素2（IL-2）和白细胞介素10（IL-10）的水平。结果显示，胚胎流产组的IL-2水平高于对照组，IL-10水平低于对照组，而胚胎流产组的IL-2/IL-10水平高于对照组。这一发现表明胚胎流产组的免疫反应受到抑制。为了进一步剖析两组阴道的微生物群，进行了16S rDNA测序。生物信息学分析表明，分别从胚胎流产组和对照组中鉴定出1096个和998个重叠的可操作分类单元。在属水平上，Fam_Finegoldia、Lac_Coprococcus_3和Lac_Roseburia的相对丰度在胚胎流产组中存在显著差异。总体而言，我们的分析为胚胎流产提供了潜在的生物标志物，阐明了微生物群与免疫反应之间的因果关系，并可能为早期流产的诊断和治疗提供可能。
