Department of Obstetrics and Gynecology, Peking Union Medical College Hospital (PUMCH), Chinese Academy of Medical Sciences (CAMS) & Peking Union Medical College, Beijing, China.
Department of Obstetrics and Gynecology, Peking Union Medical College Hospital (PUMCH), Chinese Academy of Medical Sciences (CAMS) & Peking Union Medical College, Beijing, China
Int J Gynecol Cancer. 2019 Jan;29(1):28-34. doi: 10.1136/ijgc-2018-000032.
Since other genital infections enhance HIV susceptibility by inducing inflammation and evidence suggests that the vaginal microbiome plays a functional role in the persistence or regression of high-risk human papillomavirus (HPV) infections, we investigated the relationship between the composition of the vaginal microbiota and the risk of high-risk HPV infection.
The study included 151 healthy women (65 HPV-positive and 86 HPV-negative) aged 20-65 at enrollment. Total genome DNA from samples was extracted using the hexadecyltrimethylammonium bromide (CTAB) CTAB method. The vaginal microbiota composition was determined by sequencing barcoded 16S rDNA gene fragments (V4) on Illumina HiSeq2500.
Of the 30 most abundant bacteria at the genus level, we found only six bacteria with a statistical difference between HPV-positive and HPV-negative women: , , , , , and was the predominant genus and was detected in all women, but there was no significant difference between the two groups for , , and . Furthermore, we found 26 types of bacteria with a statistical difference at the species level between the two groups. Anaerobic bacteria such as , , and were found significantly more frequently in HPV-positive women, which is the most important finding of our study.
Our findings suggest a possible role for the composition of the vaginal microbiota as a modifier of high-risk HPV infection, and specific microbiota species may serve as sensors for changes in the cervical microenvironment associated with high-risk HPV infection. The exact molecular mechanism of the vaginal microbiota in the course of high-risk HPV infection and cervical neoplasia should be further explored. Future research should include intervention in the composition of the vaginal microbiota to reverse the course of high-risk HPV infection and the natural history of cervical neoplasia.
由于其他生殖器感染通过诱导炎症而增加 HIV 易感性，并且有证据表明阴道微生物组在高危型人乳头瘤病毒（HPV）感染的持续或消退中发挥功能作用，我们研究了阴道微生物组的组成与高危 HPV 感染风险之间的关系。
本研究纳入了 151 名年龄在 20-65 岁的健康女性（65 名 HPV 阳性和 86 名 HPV 阴性）。使用十六烷基三甲基溴化铵（CTAB）CTAB 法从样本中提取总基因组 DNA。通过对 Illumina HiSeq2500 上的测序条码 16S rDNA 基因片段（V4）来确定阴道微生物组组成。
在属水平上的 30 种最丰富的细菌中，我们仅发现 HPV 阳性和 HPV 阴性女性之间存在统计学差异的 6 种细菌：、、、、和 ，是主要属，在所有女性中均有检测到，但两组之间在、、和 方面没有显著差异。此外，我们发现两组之间在种水平上有统计学差异的 26 种细菌。在 HPV 阳性女性中，发现厌氧菌如、和 等更为频繁，这是我们研究的最重要发现。
我们的研究结果表明，阴道微生物组的组成可能作为高危 HPV 感染的修饰因子发挥作用，并且特定的微生物物种可能作为与高危 HPV 感染相关的宫颈微环境变化的传感器。阴道微生物组在高危 HPV 感染和宫颈癌发病过程中的确切分子机制应进一步探讨。未来的研究应包括干预阴道微生物组组成，以逆转高危 HPV 感染和宫颈癌的自然史。
