Department of Hematology and Oncology, Nagoya University Graduate School of Medicine, Nagoya, Japan.
Departments of Hematology, Japanese Red Cross Nagoya Daiini Hospital, Nagoya, Aichi, Japan.
PLoS One. 2020 Dec 10;15(12):e0242488. doi: 10.1371/journal.pone.0242488. eCollection 2020.
Macrophages play an indispensable role in both innate and acquired immunity, while the persistence of activated macrophages can sometimes be harmful to the host, resulting in multi-organ damage. Macrophages develop from monocytes in the circulation. However, little is known about the organ affinity of macrophages in the normal state. Using in vivo imaging with XenoLight DiR®, we observed that macrophages showed strong affinity for the liver, spleen and lung, and weak affinity for the gut and bone marrow, but little or no affinity for the kidney and skin. We also found that administered macrophages were still alive 168 hours after injection. On the other hand, treatment with clodronate liposomes, which are readily taken up by macrophages via phagocytosis, strongly reduced the number of macrophages in the liver, spleen and lung.
巨噬细胞在先天免疫和获得性免疫中都发挥着不可或缺的作用，而激活的巨噬细胞的持续存在有时会对宿主造成伤害，导致多器官损伤。巨噬细胞由循环中的单核细胞发育而来。然而，在正常状态下，关于巨噬细胞对器官的亲和力知之甚少。我们使用 XenoLight DiR®进行体内成像，观察到巨噬细胞对肝脏、脾脏和肺部具有很强的亲和力，对肠道和骨髓的亲和力较弱，而对肾脏和皮肤的亲和力则很小或没有。我们还发现，注射后 168 小时，给予的巨噬细胞仍然存活。另一方面，用容易通过吞噬作用被巨噬细胞摄取的氯膦酸盐脂质体处理，强烈地减少了肝脏、脾脏和肺部的巨噬细胞数量。
