从受辐照新生小鼠脸颊组织释放的细胞外囊泡在辐射后提高了细胞存活率。

Extracellular vesicles released from irradiated neonatal mouse cheek tissue increased cell survival after radiation.

作者信息

Ariyoshi Kentaro, Hiroyama Yota, Fujiwara Naoya, Miura Tomisato, Kasai Kosuke, Nakata Akifumi, Fujishima Yohei, Ting Goh Valerie Swee, Yoshida Mitsuaki A

机构信息

Integrated Center for Science and Humanities, Fukushima Medical University, 1 Hikariga-oka, Fukushima City, Fukushima, 960-1295, Japan.

Department of Radiation Biology, Institute of Radiation Emergency Medicine, Hirosaki University, 66-1 Hon-cho, Hirosaki 036-8564, Japan.

出版信息

J Radiat Res. 2021 Jan 1;62(1):73-78. doi: 10.1093/jrr/rraa106.

DOI:10.1093/jrr/rraa106

PMID:33302296

原文链接:https://pmc.ncbi.nlm.nih.gov/articles/PMC7779349/

Abstract

Alopecia is one of the common symptoms after high-dose radiation exposure. In our experiments, neonatal mice that received 7 Gy X-ray exhibited defects in overall hair growth, except for their cheeks. This phenomenon might suggest that some substances were secreted and prevented hair follicle loss in the infant tissues around their cheeks after radiation damage. In this study, we focused on exosome-like vesicles (ELV) secreted from cheek skin tissues and back skin tissues, as control, and examined their radiation protective effects on mouse fibroblast cell lines. We observed that ELV from irradiated cheek skin showed protective effects from radiation. Our results suggest that ELV from radiation-exposed cheek skin tissue is one of the secreted factors that prevent hair follicle loss after high-dose radiation.

摘要

脱发是高剂量辐射暴露后的常见症状之一。在我们的实验中，接受7 Gy X射线照射的新生小鼠除脸颊外，整体毛发生长出现缺陷。这种现象可能表明，在辐射损伤后，某些物质被分泌出来，防止了脸颊周围婴儿组织中的毛囊丢失。在本研究中，我们重点关注从脸颊皮肤组织分泌的外泌体样囊泡（ELV），并以背部皮肤组织作为对照，检测它们对小鼠成纤维细胞系的辐射防护作用。我们观察到，来自受照射脸颊皮肤的ELV具有辐射防护作用。我们的结果表明，来自辐射暴露脸颊皮肤组织的ELV是高剂量辐射后防止毛囊丢失的分泌因子之一。